Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures

Kirstin O McDonald; Stephanie M Hughes; Indranil Basak

doi:10.1007/978-1-0716-4844-5_3

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Book chapter

Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures

Kirstin O McDonald, Stephanie M Hughes and Indranil Basak

Lysosomes: Methods and Protocols, Vol.2976, pp.25-34

Methods in molecular biology, 2976, Springer Nature

14/10/2025

DOI: https://doi.org/10.1007/978-1-0716-4844-5_3

Handle:

https://hdl.handle.net/10523/48406

Abstract

Cathepsins - metabolism

Cells, Cultured

Flow Cytometry - methods

Humans

Induced Pluripotent Stem Cells - cytology

Induced Pluripotent Stem Cells - metabolism

Lysosomes - metabolism

Neurons - cytology

Neurons - metabolism

Fluorescent molecular probes have frequently been used to monitor lysosomal health, localization, abundance, and movement through the detection of acidic organelles and lysosomal enzyme activity. Flow cytometry technology provides rapid and accurate analysis of single cells (neurons) or particles (lysosomes) in suspension through laser detection. Herein, we describe how to detect lysosomes via LysoTracker™ and Magic Red Cathepsin Activity assays in iPSC-derived human neuron cultures by flow cytometry.

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Record Identifier: 9926780551501891
Title: Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures
Creators: Kirstin O McDonald
Stephanie M Hughes
Indranil Basak
Contributors: Stephanie Hughes (Editor) - University of Otago, Biochemistry
Indranil Basak (Editor) - University of Otago, Biochemistry
Publication Details: Lysosomes: Methods and Protocols, Vol.2976, pp.25-34
Academic Unit: Biochemistry
Publisher: Springer Nature
Date published ; e-published: 14/10/2025
Comment: The published version is not available in full-text in OUR Archive. Where available, a link to the published version is provided (check the DOI and/or the Files and links section). The full-text item may be open access on the publisher's website. An earlier version of the work (such as authors' accepted manuscript following peer-review or unreviewed preprint/author's original version) may be available in the Files and links section of this record. Alternatively, readers may have subscription access to the full-text from the publisher.
Language: English
Resource Type ; Subtype: Book chapter; Research Protocol

Assessing Perturbations in Lysosome Health by Flow Cytometry in iPSC-Derived Human Neuron Cultures

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