Cationic Liposome Formulation by Microfluidics for miRNA Delivery

Matthew Reily-Bell; Rishi Shah

doi:10.1007/978-1-0716-4342-6_14

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Book chapter

Cationic Liposome Formulation by Microfluidics for miRNA Delivery

Matthew Reily-Bell and Rishi Shah

Cardiomyocytes: Methods and Protocols, Vol.2894, pp.163-172

Methods in Molecular Biology, 2894, Springer Nature, 1st ed.

20/12/2024

DOI: https://doi.org/10.1007/978-1-0716-4342-6_14

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https://hdl.handle.net/10523/48223

Abstract

Cationic

Liposomes

Microfluidics

miRNA therapeutics

Qubit

RNA protection assay

RT-qPCR

MiRNA therapeutics for treatment of cardiovascular disease face several problems with delivery. Encapsulation of miRNA therapeutics in cationic liposomes has shown potential to address many of these concerns with miRNA therapeutic delivery. Here we outline the formulation and characterization of cationic liposomes, capable of encapsulating and delivering miRNA therapeutics to AC16 cardiomyocyte cell lines, by microfluidics.

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Record Identifier: 9926778508001891
Title: Cationic Liposome Formulation by Microfluidics for miRNA Delivery
Creators: Matthew Reily-Bell
Rishi Shah
Contributors: Dhananjie Chandrasekera (Editor) - University of Otago, Physiology
Rajesh Katare (Editor) - University of Otago, Physiology
Publication Details: Cardiomyocytes: Methods and Protocols, Vol.2894, pp.163-172
Academic Unit: Physiology
Publisher: Springer Nature
Date published ; e-published: 20/12/2024
Comment: The published version is not available in full-text via OUR Archive. Where available, a link to the published version is provided (check the DOI and/or the Files and links section). The full-text item may be open access on the publisher's website. An earlier version of the work (such as authors' accepted manuscript following peer-review or unreviewed preprint/author's original version) may be available in the Files and links section of this record. Alternatively, readers may have subscription access to the full-text from the publisher.
Language: English
Resource Type ; Subtype: Book chapter; Research Protocol

Cationic Liposome Formulation by Microfluidics for miRNA Delivery

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