Abstract
The brain reward circuit is complex and it plays an important role in reward-motivated behavior and is fundamental for species survival. It is also implicated in several diseases that involve motivational deficits such as depression and schizophrenia. Due to its complex circuitry involving differential sub-neuronal populations and glia, traditional lesioning, and pharmacological manipulations have failed to differentiate the effect of these complex, intermingled circuitry. Designer receptors exclusively activated by designer drugs (DREADDs) provide a minimally invasive option to circumvent these issues. In this chapter, we address the method by which DREADD is applied in behavioral neuroscience to explore the brain reward circuit in our laboratory. We highlight the potential difficulties faced by new users of this technology and provide some of our methods to overcome them. When used properly, the DREADD system is a versatile tool to interrogate circuits and brain areas associated with reward-motivated behavior and thus is a valuable addition to any behavioral neuroscientist’s methodological toolbox.