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Characterising the gut microbiomes of two baleen whales with different feeding and migratory strategies
 

Characterising the gut microbiomes of two baleen whales with different feeding and migratory strategies

Richard O'Rorke, Emma L. Carroll, Els Vermeulen, Robert Harcourt, Rochelle Constantine, Stephanie Plön, William Rayment Anthony Chariton
Zenodo
02/02/2026
:
https://hdl.handle.net/10523/49936
The data here consists of bacterial 16S rDNA metabarcoding results from Bryde's whale and southern right whale faecal samples.  The dataset includes the following files: • metadata.csv - metadata associated with whale faecal samples. Input file for whale-microbiome.R.  • metadata-noreps.csv - metadata associated with whale faecal samples, same file as above except technical replicates of samples have been combined for some analyses. Input file for whale-microbiome.R.  • 16S-OTU.csv - OTU table of 16S metabarcoding results. Input file for whale-microbiome.R.  • 16S-OTU-noreps.csv - OTU table of 16S metabarcoding results as above, however, technical replicates of biological samples have been combined by averaging their reads. Input file for whale-microbiome.R.   • 16S-tree-NJ.newick - Neighbour-Joining phylogenetic tree constructed to examine phylogenetic distances between microbial communities of different whales. Input file for whale-microbiome.R.  • EC.tsv - PICRUSt2 output file with Enzyme Commission numbers mapped to 16S rRNA sequences to predict functionality. Input file for whale-microbiome.R.  • ec-table.csv - mapping file to match EC numbers to their names. Input file for whale-microbiome.R.  • Supplementary_Information.docx - supplementary figures and tables associated with main research article.  The R code whale-microbiome.R is available from https://github.com/aashiparikh/Baleen-whale-gut-microbiomes. This is the code for statistical analyses including alpha and beta diversity, core microbiome and functional analyses performed on 16S metabarcoding results to examine baleen whale gut microbiomes.   

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url
https://doi.org/10.5281/zenodo.18454775
Data
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