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A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus
Journal article   Peer reviewed

A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus

Peter Reid, Nicholas C.K. Heng, John D. Hale, Deepti Krishnan, Julian Crane, John R. Tagg and Trudy J. Milne
Journal of microbiological methods, Vol.170, pp.105837-105837
03/2020
DOI: https://doi.org/10.1016/j.mimet.2020.105837
Handle:
https://hdl.handle.net/10523/22549

Abstract

Salivaricin B Streptococcus salivarius K12 TaqMan™ quantitative PCR assay
In order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations targeting genes of the locus encoding the lantibiotic bacteriocin salivaricin B.

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