Abbott RealTime HIV-1 m2000rt viral load testing: Manual extraction versus the automated m2000sp extraction

Lesley E. Scott; John A. Crump; Emma Msuya; Anne B. Morrissey; Willem F. Venter; Wendy S. Stevens

doi:10.1016/j.jviromet.2010.12.006

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Abbott RealTime HIV-1 m2000rt viral load testing: Manual extraction versus the automated m2000sp extraction

Journal article

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Abbott RealTime HIV-1 m2000rt viral load testing: Manual extraction versus the automated m2000sp extraction

Lesley E. Scott, John A. Crump, Emma Msuya, Anne B. Morrissey, Willem F. Venter and Wendy S. Stevens

Journal of virological methods, Vol.172(1-2), pp.78-80

01/03/2011

DOI: https://doi.org/10.1016/j.jviromet.2010.12.006

Abstract

Biochemical Research Methods

Biochemistry & Molecular Biology

Biotechnology & Applied Microbiology

Life Sciences & Biomedicine

Science & Technology

Virology

The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually. (C) 2010 Elsevier B.V. All rights reserved.

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Record Identifier: 9926517714001891
Title: Abbott RealTime HIV-1 m2000rt viral load testing: Manual extraction versus the automated m2000sp extraction
Creators: Lesley E. Scott
John A. Crump
Emma Msuya
Anne B. Morrissey
Willem F. Venter
Wendy S. Stevens
Publication Details: Journal of virological methods, Vol.172(1-2), pp.78-80
Academic Unit: Centre for International Health
Publisher: Elsevier
Grant note: U19AI067854 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) AI 062563 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA D43TW006732 / FOGARTY INTERNATIONAL CENTER; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Fogarty International Center (FIC) U01 AI067854 / Center for HIV/AIDS Vaccine Immunology, a United States National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA U01 AI069484-01 / Duke Clinical Trials Unit and Clinical Research Sites
Date published ; e-published: 01/03/2011
Language: English
Resource Type: Journal article

Abbott RealTime HIV-1 m2000rt viral load testing: Manual extraction versus the automated m2000sp extraction

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