Abstract
Brain-derived neurotrophic factor (BDNF) is implicated in neuronal differentiation, maturation, survival, and synaptic plasticity. Circulating BDNF is often quantified to represent brain neuronal plasticity following acute exercise. However, BDNF in the fluid compartment surrounding the brain has not been quantified in healthy humans following exercise. We measured total and free BDNF in arterial and internal jugular venous blood to assess cross-brain BDNF release, and in cerebrospinal fluid (CSF) via lumbar puncture, before and following ~2 hours of cycling exercise. We hypothesized that exercise would elevate both systemic circulating BDNF and CSF BDNF concentrations. Herein, in humans, we demonstrate that BDNF in the CSF is elevated following exercise. We also report a net release of BDNF from the brain into circulation despite being several orders of magnitude less concentrated in the CSF.