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Extracellular RNA Isolation from Cell Culture Supernatant
Journal article

Extracellular RNA Isolation from Cell Culture Supernatant

Sawen Bakr, Bridget Simonson, Kirsty M Danielson and Saumya Das
Methods in molecular biology (Clifton, N.J.), Vol.1740, pp.23-34
2018
DOI: https://doi.org/10.1007/978-1-4939-7652-2_3
Handle:
https://hdl.handle.net/10523/39220

Abstract

Animals Cell Culture Techniques Chemical Precipitation Extracellular Vesicles - metabolism Filtration - methods Humans Molecular Biology - methods RNA - analysis RNA - isolation & purification Ultracentrifugation - methods
Extracellular RNAs are emerging as novel biomarkers and mediators of intercellular communication. Various methods to isolate RNA from biofluids and cell culture supernatants have been previously used by investigators. Here, we describe several standardized protocols for the isolation of RNAs from cell culture supernatants that utilize commercially available kits and reagents.

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