Fabrication and Characterization of Silk-Fibroin, Polyvinyl Alcohol, and Natural Compounds-Derived Bioscaffold to Accelerate Wound Healing

Shubhra Sinha; Jekhan Andimadam Madana Saravanan; Azam Ali; Rajesh Katare

doi:10.31083/FBL50459

Fabrication and Characterization of Silk-Fibroin, Polyvinyl Alcohol, and Natural Compounds-Derived Bioscaffold to Accelerate Wound Healing

Shubhra Sinha, Jekhan Andimadam Madana Saravanan, Azam Ali Rajesh Katare

Frontiers in bioscience (Landmark Ed.), Vol.31(5), 50459

25/05/2026

: https://doi.org/10.31083/FBL50459

https://hdl.handle.net/10523/51131

Manuka honey

angiogenesis

fenugreek seeds

ghrelin

nanofibers

reactive oxygen species

wound healing

Background: Impaired wound healing represents a significant clinical challenge. Persistent inflammation, excessive reactive oxygen species, and impaired angiogenesis are key mechanisms underlying nonhealing ulcers. We developed a multifunctional bioscaffold incorporating natural bioactive compounds as a mechanistic therapeutic strategy. Methods: Polyvinyl alcohol and silk fibroin formed the scaffold matrix, blended with Manuka honey, fenugreek seed extract, and Ghrelin for their antioxidant and proangiogenic properties. Results: Scanning electron microscopy revealed defect-free nanofibers with diameters below 200 nm. Fourier-transform infrared analysis confirmed successful incorporation of the bioactive components, indicating effective interactions within the polyvinyl alcohol (PVA)-silk fibroin (SF) matrix. Thermal analysis demonstrated improved thermal stability upon addition of the bioactive agents. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed the absence of toxicity. Scratch assays confirmed enhanced migration of human umbilical vein endothelial cells, supporting the scaffold's angiogenic potential. Additionally, the scaffold exhibited superior antioxidant activity and significantly improved cell proliferation. Conclusions: These findings demonstrate that the developed bioscaffold integrates antioxidant and proangiogenic properties, making it a promising candidate for various biomedical applications, particularly in wound dressings, drug delivery, tissue engineering, and biosensing.

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https://doi.org/10.31083/FBL50459

Published (Version of record)

: 9926870088201891
: Fabrication and Characterization of Silk-Fibroin, Polyvinyl Alcohol, and Natural Compounds-Derived Bioscaffold to Accelerate Wound Healing
: Shubhra Sinha
Jekhan Andimadam Madana Saravanan
Azam Ali
Rajesh Katare
: Bioengineering; Oral Rehabilitation; Sir John Walsh Research Institute; Physiology
: Frontiers in bioscience (Landmark Ed.), Vol.31(5), 50459
: IMR Press
: 25/05/2026
: Cell free synthetic exosomes incorporated nanomatrix for the treatment of ischaemic diabetic ulcer, UOOX2205, Ministry of Business, Innovation and Employment (New Zealand, Wellington) - MBIE
: New Zealand Heart Foundation Doctoral Scholarship (PJ-0000154)
: Copyright © The Author(s) 2026. This work was first published in Frontiers in Bioscience (Landmark Edition) (IMR Press). This is an open access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://www.creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, provided that the original work is properly attributed to the creator(s) and the source, a link to the Creative Commons license is provided, and any changes made are indicated.
: English
: Journal article