Abstract
Bloodstream infections induce considerable morbidity, high mortality, and represent a significant burden of cost in health care; however, our understanding of the immune response to bacteremia is incomplete. Langerin
+
CD8α
+
dendritic cells (DCs), residing in the marginal zone of the murine spleen, have the capacity to cross-prime CD8
+
T cells and produce IL-12, both of which are important components of antimicrobial immunity. Accordingly, we hypothesized that this DC subset may be a key promoter of adaptive immune responses to blood-borne bacterial infections. Utilizing mice that express the diphtheria toxin receptor under control of the langerin promoter, we investigated the impact of depleting langerin
+
CD8α
+
DCs in a murine model of intravenous infection with
Mycobacterium bovis
bacille Calmette–Guerin (BCG). In the absence of langerin
+
CD8α
+
DCs, the immune response to blood-borne BCG infection was diminished: bacterial numbers in the spleen increased, serum IL-12p40 decreased, and delayed CD8
+
T cell activation, proliferation, and IFN-γ production was evident. Our data revealed that langerin
+
CD8α
+
DCs play a pivotal role in initiating CD8
+
T cell responses and IL-12 production in response to bacteremia and may influence the early control of systemic bacterial infections.