Protocol for a murine skin abscess model to study bacterial infection dynamics, microbial interactions, and treatment efficacy

Anupriya Gupta; Kathleen J. Sircombe; Janaya D. Stevenson; Samuel J.T. Wardell; Daniel Pletzer

doi:10.1016/j.xpro.2025.104291

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Protocol for a murine skin abscess model to study bacterial infection dynamics, microbial interactions, and treatment efficacy

Journal article

Open access

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Protocol for a murine skin abscess model to study bacterial infection dynamics, microbial interactions, and treatment efficacy

Anupriya Gupta, Kathleen J. Sircombe, Janaya D. Stevenson, Samuel J.T. Wardell and Daniel Pletzer

STAR protocols, Vol.7(1), 104291

20/12/2025

DOI: https://doi.org/10.1016/j.xpro.2025.104291

Handle:

https://hdl.handle.net/10523/49354

Abstract

health sciences

immunology

microbiology

model organisms

We present a murine skin abscess model to study pathogen-pathogen and host-pathogen interactions, infection progression, and therapeutic interventions. We outline steps for real-time monitoring of bacterial infection dynamics and host responses using bioluminescent or fluorescently labeled bacteria using IVIS (in vivo imaging system). We provide details to isolate bacterial and host RNA/DNA from infected tissues, enabling downstream transcriptomic and molecular analyses. This model offers a robust platform to study bacterial pathogenesis and host immunity and evaluate antimicrobial or innovative treatment strategies. For complete details on the use and execution of this protocol, please refer to Pletzer et al. https://doi.org/10.1128/mBio.00140-17.

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Record Identifier: 9926823821901891
Title: Protocol for a murine skin abscess model to study bacterial infection dynamics, microbial interactions, and treatment efficacy
Creators: Anupriya Gupta
Kathleen J. Sircombe
Janaya D. Stevenson
Samuel J.T. Wardell
Daniel Pletzer
Publication Details: STAR protocols, Vol.7(1), 104291
Academic Unit: Microbiology and Immunology
Publisher: Elsevier
Grants: Molecular mechanisms underlying pathogen-pathogen interaction during skin infection, 22-UOO-005, Royal Society Te Apārangi (New Zealand, Wellington)
Grant note: University of Otago Doctoral Scholarship; Te Niwha MSc Scholarship (TN/PLSM/24/48/UOOJS, AP41789_CON26674); University of Otago Research Grant
Date published ; e-published: 20/12/2025
Copyright: Copyright © The Author(s) 2025. This work was first published in STAR Protocols (Elsevier). This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://www.creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, provided that the original work is properly attributed to the creator(s) and the source, a link to the Creative Commons license is provided, and any changes made are indicated.
Language: English
Resource Type ; Subtype: Journal article; Research Protocol