Abstract
Procymidone is frequently detected in vegetables, prompting stringent regulatory limits in many countries and regions. Despite this, the development of rapid and sensitive detection methods remains limited. In this study, an aptamer-based lateral flow assay (LFA) was developed for procymidone detection, utilizing Prussian blue nanoparticles (PBNPs) as amplified signal markers, chosen for their intrinsic color and enzyme-like properties. An aptamer was coupled with the PBNPs to form a signal probe, enabling the construction of a competitive LFA. Under optimized conditions, the LFA achieved a limit of detection (LOD) of 0.35ng/mL and a visual limit of detection (vLOD) of 0.64ng/mL. The enzyme-mimetic activity of PBNPs was harnessed and such a catalytic reaction strategy further improved the assay sensitivity, leading to a further improvement of the LOD by 14.3-fold. The assay demonstrated high sensitivity, selectivity, and reproducibility, performing effectively in typical vegetable samples. This study provides a rapid, sensitive and reliable tool for procymidone detection, offering valuable insights and a new strategy for monitoring pesticides.
• Prussian blue nanoparticles (PBNPs) were applied as signal amplifiers to enhance the colorimetric signals in an aptamer-based LFA for procymidone.
• The LOD of the LFA were improved by 14.3-fold, through enzymatic catalytic amplification.
• The developed LFA demonstrated high selectivity and sensitivity for detecting procymidone in vegetable samples.
• The LFA exhibited excellent selectivity, showing negligible cross-reactivity with structural and functional analogues of procymidone.