Abstract
The effect of pulsed electric field (PEF) on in-vitro simulated gastrointestinal protein digestion of cold-boned deer Longissimus dorsi was elucidated. PEF treated samples viz. T1 (2.5 kV, 50 Hz) and T2 (10 kV, 90 Hz) along with a control were subjected to in-vitro simulated gastrointestinal protein digestion. Samples were collected after 0, 30, and 60 min of gastric digestion and 120 and 180 min of intestinal digestion. A significant (P < 0.05) increase was observed in the protein digestibility and soluble protein (%) of PEF treated samples, highlighting a positive influence of PEF processing on the digestion process. Higher concentrations of almost all the free amino acids were observed for the PEF treated samples. No significant effect of PEF was observed on the release of various minerals. PEF may be utilized for the development of novel muscle foods with improved protein digestibility.
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•PEF processing increased (P < 0.05) the digestibility of the muscle during in-vitro simulated gastrointestinal digestion.•Higher (P < 0.05) soluble protein (%) was observed for PEF-processed samples.•PEF-processed samples showed higher values for free amino acids.•Pulsed electric field had no impact on the release of minerals during in-vitro gastrointestinal digestion.