Abstract
The effects of pH, ionic strength and polyvalent ions upon the subsequent adherence of
E. coli to octyl-sepharose in hydrophobic interaction chromatography (HIC), and the effect of polyvalent ions on adherence to dichloromethane in the bacterial adherence to hydrocarbons (BATH) tests were investigated. Ionic strength, pH and polyvalent ions were found to influence adhesion. Lowering pH and increasing ionic strength increased adherence of
E. coli to octyl-Sepharose and dichloromethane. However, polyvalent ions increased adherence to octyl-Sepharose while reducing adherence to dichloromethane, indicating the possibility of different mechanisms of interaction between
E. coli and these substrates.