Abstract
Darwin's ant, Doleromyrma darwiniana, is a native ant to Australia that has established invasive populations in nearby Aotearoa, New Zealand. Darwin's ant belongs to the Dolichoderinae subfamily of the Formicidae family, which contains species with a broad range of invasion histories, including the internationally prolific Argentine ant (Linepithema humile). The genome of Darwin's ant, Doleromyrma darwiniana, is presented here, created from a combined sample of 10 female workers. Generated using Oxford Nanopore Technologies PromethION 2 sequencing data, the assembly has a total length of 238.80 Mb and an N50 of 14.23 Mb. The assembly is compared to that of L. humile and four additional species from the same subfamily, the Dolichoderinae. Annotations were generated for all six Dolichoderinae species and sorted into orthogroups to assess the diversity and conservation of the Dolichoderinae genomic repertoire.