Is the immunogenicity of PD-1 blocking antibodies a confounding variable in murine studies?

Linda A Buss; Gabi U Dachs; Liping Goddard; Abel D Ang; Bridget A Robinson; Margaret J Currie; Barry Hock

doi:10.1016/j.imlet.2021.04.003

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Is the immunogenicity of PD-1 blocking antibodies a confounding variable in murine studies?

Letter/Communication

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Is the immunogenicity of PD-1 blocking antibodies a confounding variable in murine studies?

Linda A Buss, Gabi U Dachs, Liping Goddard, Abel D Ang, Bridget A Robinson, Margaret J Currie and Barry Hock

Immunology letters, Vol.234, pp.13-15

13/04/2021

DOI: https://doi.org/10.1016/j.imlet.2021.04.003

Handle:

https://hdl.handle.net/10523/18008

Abstract

PD-1

Mackenzie Cancer Research Group

Haematology Research Group

mouse studies

confounding factors

immunogenicity

anti-drug immune responses

While conducting exercise oncology experiments with anti-PD-1 (rat-derived; Bio-X-Cell, BE0146) in C57BL/6 mice with B16-F10 melanoma or EO771 breast tumours, we noted that results from isotype control (rat IgG2a; Bio-X-Cell, BE0089)-treated mice appeared to be substantially different from those in untreated mice from a prior study. This led us to postulate that the immunogenicity of the rat mAb was having unintended effects on the anti-tumour immune response. Hence, we investigated the levels of anti-rat antibodies in the plasma of untreated, IgG2a-treated and anti-PD-1-treated mice.

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Record Identifier: 9926495352101891
Title: Is the immunogenicity of PD-1 blocking antibodies a confounding variable in murine studies?
Creators: Linda A Buss
Gabi U Dachs
Liping Goddard
Abel D Ang
Bridget A Robinson
Margaret J Currie
Barry Hock
Publication Details: Immunology letters, Vol.234, pp.13-15
Academic Unit: Pathology and Molecular Medicine (UOC)
Publisher: Elsevier
Grant note: Mackenzie Charitable Foundation; University of Otago
Date published ; e-published: 13/04/2021
Comment: The published version is not available in full-text in OUR Archive. Where available, a link to the published version is provided (check the DOI and/or the Files and links section). The full-text item may be open access on the publisher's website. An earlier version of the work (such as authors' accepted manuscript following peer-review or unreviewed preprint/author's original version) may be available in the Files and links section of this record. Alternatively, readers may have subscription access to the full-text from the publisher.
Language: English
Resource Type ; Subtype: Letter/Communication

Is the immunogenicity of PD-1 blocking antibodies a confounding variable in murine studies?

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