Show simple item record

dc.contributor.advisorCook, Gregory
dc.contributor.authorMcKay Vercoe, Reuben Blake
dc.date.available2020-05-28T03:53:49Z
dc.date.copyright2020
dc.identifier.citationMcKay Vercoe, R. B. (2020). Ion homeostasis in Mycobacterium smegmatis (Thesis, Doctor of Philosophy). University of Otago. Retrieved from http://hdl.handle.net/10523/10095en
dc.identifier.urihttp://hdl.handle.net/10523/10095
dc.description.abstractMicroorganisms must maintain equilibrium and intracellular availability of ions for growth and persistence in a range of environments, either in the face of scarce resources or excesses, whereby they must buffer, exclude or export these ions preventing accumulation to harmful levels. Ion homeostasis is essential for energy generation, metabolism, regulation of internal pH and osmotic homeostasis. Mycobacteria are part of the native microflora found in soils and aquatic environments and harbour an extensive repertoire of uptake and efflux and secretion pathways to modulate ions and metabolites including, sodium, potassium, calcium, magnesium, iron, nickel, manganese, zinc, copper and cobalt. There remains a paucity of information regarding ion homeostasis networks and pathways in mycobacteria. The overarching aim of this thesis was to characterise ion homeostasis and energetics in the saprophyte Mycobacterium smegmatis, under normoxia and hypoxia, under ion chelation and deficiency, with heavy-metal exposure and in response to anti-tubercular compounds with putative disruptive effects on ion energetics, including bedaquiline and amiloride derivatives. Inductively coupled plasma mass spectroscopy (ICP-MS) analysis of wild-type M. smegmatis and energy-driven ion transport mutants revealed the molecular inventory of cations in M. smegmatis under normoxia and hypoxia. The intracellular cation profile of M. smegmatis was comprised primarily of sodium (40-85%), potassium (10-55%) and magnesium (5-7%), while trace elements consisting of calcium, iron, nickel, manganese, zinc, copper and cobalt were less than 1.5% of the profile. ICP-MS analysis also demonstrated changes in ion homeostasis from disruptions made using chelation agents to reduce the availability of manganese, iron, cobalt and zinc. Cultures exposed to 2 mM nickel and manganese resulted in intracellular accumulation of these cations and significant depletion of intracellular cobalt, iron, magnesium and potassium concentrations. Disruption of potassium homeostasis in the ΔtrkA mutant of M. smegmatis resulted in the reduction of (~10-50%) intracellular potassium concentrations and elevated (~10-30%) intracellular sodium concentrations, coupled with reductions in viability and persistence under hypoxia. This study has demonstrated the molecular inventory of intracellular ions in M. smegmatis under several key laboratory conditions and has characterised essential components of ion homeostasis and more specifically, potassium homeostasis and energetics in mycobacteria. The role of several energy-driven transport systems and the effects of anti- tubercular compounds on cellular and ion homeostasis in M. smegmatis and Mycobacterium tuberculosis were also studied.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectNew Zealand
dc.subjectPotassium
dc.subjectMycobacterium smegmatis
dc.subjectcation homeostasis
dc.subjectMolecular Microbiology
dc.titleIon homeostasis in Mycobacterium smegmatis
dc.typeThesis
dc.date.updated2020-05-27T23:29:29Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameDoctor of Philosophy
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.interloanno
otago.openaccessAbstract Only
otago.evidence.presentYes
otago.abstractonly.term26w
 Find in your library

Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item is not available in full-text via OUR Archive.

If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.

This item appears in the following Collection(s)

Show simple item record