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dc.contributor.advisorBostina, Mihnea
dc.contributor.authorJayawardena, Gimshan Nadishka
dc.date.available2020-06-30T23:25:36Z
dc.date.copyright2020
dc.identifier.citationJayawardena, G. N. (2020). Structural insights into oncolytic Seneca Valley Virus capsid and receptor interactions (Thesis, Doctor of Philosophy). University of Otago. Retrieved from http://hdl.handle.net/10523/10154en
dc.identifier.urihttp://hdl.handle.net/10523/10154
dc.description.abstractOncolytic viruses (OVs) are replication competent agents that selectively target cancer cells. Seneca Valley Virus (SVV) is a newly-discovered oncolytic picornavirus which is classified as the sole member in the genus Senecavirus. SVV strain 001 has currently completed Phase I and Phase II clinical trials in pediatric solid tumors and small-cell lung cancer, respectively. Similar to other picornaviruses, SVV forms naturally occurring empty capsids (without genome), known as procapsids, which share the same antigenicity as full virions. Understanding the formation and structure of SVV procapsids could give insights into how to exploit them as virus-like particles (VLPs) for targeted in vivo drug delivery for cancer treatment. Recently, we identified the Anthrax Toxin Receptor 1 (ANTXR1), a membrane protein overexpressed in ~60% of types of cancer, as the high-affinity cellular recept or for SVV in cancer cells. However, the high-resolution information on SVV-ANTXR1 interaction sites remained poorly characterized, thereby hampering the potential to develop SVV mutant in future oncovirotherapy. In this study, we purified SVV full capsids, procapsids and used cryo-electron microscopy (cryo-EM) to investigate the capsid architectures and receptor interactions. Our results show that both SVV full capsids and procapsids have a similar external structure, while on the interior the main differences were the missing genomic RNA in the procapsid and a disordered VP1 region. We also show that a cage of RNA serves to stabilize the inside surface of the full capsid, thereby making it more acid stable. In an attempt to improve SVV particle stability, we isolated a thermostable mutant of SVV by thermal selection assays. Cryo-EM analysis of SVV-56mt structure showed conformational shifts at the interpentameric surface that facilitated the formation of additional hydrogen bonds and aromatic interactions, which could serve as capsid stabilizing factors. In SVV-ANTXR1 complex, ANTXR1 decorates the outer surface of the SVV capsid and interacts with the surface exposed BC loop and loo p II of VP1, “the puff” of VP2 and “the knob” of VP3. Comparison of the receptor-bound capsid structure with the native capsid structure reveals that receptor binding induces minor conformational changes in SVV capsid structure, suggesting the role of ANTXR1 as an attachment receptor. Our results demonstrate that the capsid footprint on the receptor is not conserved in anthrax toxin receptor 2 (ANTXR2), thereby providing a molecular mechanism for explaining the exquisite selectivity of SVV for ANTXR1. Findings from this study lay the foundation for the modification of the SVV procapsid to develop it for targeted in vivo delivery of therapeutics and to develop potent SVV mutants with specific cancer tropism.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectNew Zealand
dc.subjectSVV
dc.subjectVirus
dc.subjectCancer
dc.subjectCryo-EM
dc.subjectANTXR1
dc.titleStructural insights into oncolytic Seneca Valley Virus capsid and receptor interactions
dc.typeThesis
dc.date.updated2020-06-30T04:59:42Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameDoctor of Philosophy
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.interloanno
otago.openaccessAbstract Only
otago.evidence.presentYes
otago.abstractonly.term1y*
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