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dc.contributor.advisorSummerfield, Tina
dc.contributor.authorLim, Grace
dc.date.available2020-11-10T20:31:16Z
dc.date.copyright2020
dc.identifier.citationLim, G. (2020). Investigation of the Genes sll1354, sll1165, and sll1520 in Genomic Instability in Cyanobacterium Synechocystis sp. PCC 6803 (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/10523en
dc.identifier.urihttp://hdl.handle.net/10523/10523
dc.description.abstractThe model cyanobacterium Synechocystis sp. PCC 6803 is used extensively in the studies of oxygenic photosynthesis as well as genetic engineering. The ability to incorporate exogenous DNA into its genome through homologous recombination makes it a potential biosystem that can be manipulated to produce valuable compounds. Synechocystis sp. PCC 6803 possesses an advanced DNA recombination and repair mechanism that enables the survival of the bacterium in various environments. This mechanism serves as a double edge sword as it improves the plasticity of the cyanobacterium; on the other hand, it also promotes genomic instability. This study investigated the role of the genes sll1354 (recJ), sll1165 (mutS1), and sll1520 (recN) in genomic instability. A PS II mutant strain showed signs of genomic instability where it grew photoautotrophically in pH 7.5 medium, which it is incapable of growing initially. When the constructs of interrupted genes of interest were introduced into the strain, the mutants' ability to grow photoautotrophically in pH 7.5 medium was compared to the control. The interruption of sll1354 and sll1520 was found to show a minimal effect on the growth rate reversion, whereas disruption on sll1165 was found to restored photoautotrophic growth in pH7.5 medium. This preliminary data in this study suggested that the gene sll1165 may be one of the genes that is involved in the DNA recombination and repair mechanism in Synechocystis sp. PCC 6803, which, in turn, may plays a role in cyanobacterial genomic instability.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectSynechocystis sp. PCC 6803
dc.subjectDNA recombination and repair mechanism
dc.subjectgenomic instability
dc.subjectRecJ
dc.subjectMutS1
dc.subjectRecN
dc.titleInvestigation of the Genes sll1354, sll1165, and sll1520 in Genomic Instability in Cyanobacterium Synechocystis sp. PCC 6803
dc.typeThesis
dc.date.updated2020-11-09T21:20:39Z
dc.language.rfc3066en
thesis.degree.disciplineDepartment Of Botany
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.openaccessOpen
otago.evidence.presentYes
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