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dc.contributor.authorChiu, Jasper
dc.contributor.authorTucker, Ian
dc.contributor.authorMcDowell, Arlene
dc.contributor.authorMcLeod, Bernie
dc.date.available2021-09-14T20:34:10Z
dc.date.copyright2014
dc.identifier.citationChiu JZS, Tucker IG, McLeod BJ and McDowell A (2015). Arginine-tagging of polymeric nanoparticles via histidine to improve cellular uptake. European Journal of Pharmaceutics and Biopharmaceutics 89: 48-55en_NZ
dc.identifier.urihttp://hdl.handle.net/10523/12265
dc.description.abstractPolyarginine, a cell-penetrating peptide, has been shown to aid cellular penetration of bioactives into cells. We utilized a novel approach of using a histidine linker to produce poly(ethyl-cyanoacrylate) (PECA) nanoparticles tagged with oligoarginine and investigated cellular uptake. MALDI TOF/TOF (tandem) analysis revealed that di-arginine-histidine (RRH) covalently bound to PECA nanoparticles to form cationic particles (+18 mV), while longer oligoarginine peptides did not co-polymerize with PECA nanoparticles. Although RRH-tagged nanoparticles had similar size and FITC-dextran entrapment efficiency compared to unmodified nanoparticles, RRH-tagging of nanoparticles resulted in a greater release of FITC-dextran. As the nanoparticles were found to aggregate in Hanks Balanced Salt Solution (HBSS), the effect of phosphate on the zeta-potential of nanoparticles was studied. Treating the nanoparticles with poloxamer-407 prevented aggregation. RRH-tagged PECA nanoparticles increased cellular uptake by a further 30% compared to unmodified PECA nanoparticles and was concentration dependent. We suggest that enhanced cell uptake can be achieved using a di-arginine histidine construct as opposed to the previously published findings that a minimum of hexa-arginine is necessary. Further, the cationic zeta-potential of the cell-penetrating peptide may not be needed to enhance uptake.en_NZ
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dc.format.mimetypeapplication/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.language.isoenen_NZ
dc.publisherElsevieren_NZ
dc.relation.ispartofEuropean Journal of Pharmaceutics and Biopharmaceuticsen_NZ
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPoly(ethyl-cyanoacrylate) nanoparticlesen_NZ
dc.subjectpolyarginineen_NZ
dc.subjectMALDI-TOFen_NZ
dc.subjectcationicen_NZ
dc.subjectcell-penetrating peptidesen_NZ
dc.titleArginine-tagging of Polymeric Nanoparticles Via Histidine to Improve Cellular Uptakeen_NZ
dc.typeJournal Articleen_NZ
dc.date.updated2021-09-14T05:13:17Z
otago.schoolSchool of Pharmacyen_NZ
otago.relation.volume89en_NZ
dc.identifier.doi10.1016/j.ejpb.2014.11.014en_NZ
otago.bitstream.endpage55en_NZ
otago.bitstream.startpage48en_NZ
otago.openaccessOpenen_NZ
dc.rights.statementThis version in OUR Archive is the author’s manuscript accepted for publication after peer-review. The published version is: Chiu JZS, Tucker IG, McLeod BJ and McDowell A (2015). Arginine-tagging of polymeric nanoparticles via histidine to improve cellular uptake. European Journal of Pharmaceutics and Biopharmaceutics 89: 48-55 This OUR Archive version is licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/en_NZ
dc.description.refereedPeer Revieweden_NZ
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Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International