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dc.contributor.advisorBhatia, Madhav
dc.contributor.authorCampbell, Elizabeth Julia
dc.date.available2011-11-30T20:43:48Z
dc.date.copyright2011
dc.identifier.citationCampbell, E. J. (2011). Hydrogen Sulfide: Investigation into the Effects of Different H2S Donors on the Upregulation of Inflammatory Cytokines (Thesis, Bachelor of Biomedical Sciences with Honours). University of Otago. Retrieved from http://hdl.handle.net/10523/2052en
dc.identifier.urihttp://hdl.handle.net/10523/2052
dc.description.abstractEndogenous hydrogen sulfide (H2S) has recently been described as the third gaseous mediator of interest. It has been proposed in the literature [1] , that H2S has a role in inflammation, through the induction of upstream signals, which activate inflammatory mediators such as TNF-α, IL-1β and IL-6, and through this method H2S can give rise to a pro-inflammatory response. This study, therefore, investigated the mechanisms by which H2S contributes to inflammation in vitro, through the use of two sulfide donors, sodium hydrosulfide (NaHS) and sodium sulfide (Na2S), to look at the inflammatory mediator release from differentiated U937 monocytic cells. Through the use of RT-PCR and ELISA experimentation, analysis was carried out using the two sulfide donors at different concentrations and time points. The results found, that there was no difference between the two sulfide donors in the upregulation of inflammatory cytokines. It was also found that the effects of concentration and time were significant in ELISA experiments, but not so with the PCR experiments. However, when either of these compounds were added into an in vitro system using differentiated U937 cells, there was an upregulation of both protein and mRNA expression relative to the untreated control group.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectHydrogen Sulfide
dc.subjectInflammation
dc.titleHydrogen Sulfide: Investigation into the Effects of Different H2S Donors on the Upregulation of Inflammatory Cytokines
dc.typeThesis
dc.date.updated2011-11-29T21:45:41Z
thesis.degree.disciplinePathology
thesis.degree.nameBachelor of Biomedical Sciences with Honours
thesis.degree.grantorUniversity of Otago
thesis.degree.levelHonours
otago.interloanyes
otago.openaccessAbstract Only
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