Show simple item record

dc.contributor.advisorEaton-Rye, Julian
dc.contributor.authorMoore, Jonathan
dc.date.available2011-12-14T22:25:42Z
dc.date.copyright2011
dc.identifier.citationMoore, J. (2011). The Psb28 Proteins of Oxygenic Photosynthesis (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/2073en
dc.identifier.urihttp://hdl.handle.net/10523/2073
dc.description.abstractThis project focused on the Photosystem II (PS II) extrinsic proteins Psb28 and Psb28-2 and reports, for the first time, results using a mutant deficient in Psb28-2. The psb28 and psb28-2 genes were interrupted with antibiotic-resistance cassettes both singularly and in combination together. The resulting strains: Dpsb28, DPsb28-2 and Dpsb28:DPsb28-2 along with a wild-type control strain were physiologically tested to determine photoautotrophic growth rates; oxygen evolution rates; vulnerability to, and ability to recover from, photoinhibition; relative quantities of PSII; fluorescence characteristics at liquid nitrogen temperatures; and the expression of psb28 and psb28-2. The two single mutants (Dpsb28 and DPsb28-2) were found to grow slightly slower than wild type and the double mutant (Dpsb28:DPsb28-2) was found to grow slower again. No substantial differences were found in oxygen evolution rates, ability to recover from and susceptibility to photoinhibition or the relative quantities of PSIIbetween mutants and wild type. The use of 77 K fluorescence was developed during this project and was subsequently used to demonstrate that the mutants lacking Psb28-2 have deficient energy transfer between the phycobilisomes and the photosystems, thus providing the first indication of separate functions of the Psb28 and Psb28-2 proteins. This project has laid the groundwork for further research into Psb28 and Psb28-2, and the Dpsb28, DPsb28-2 and Dpsb28:DPsb28-2 strains created in this project will provide the tools for further research into these proteins.en_NZ
dc.format.mimetypeapplication/pdf
dc.language.isoenen_NZ
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectPhotosystem IIen_NZ
dc.subjectPsb28en_NZ
dc.titleThe Psb28 Proteins of Oxygenic Photosynthesisen_NZ
dc.typeThesis
dc.date.updated2011-12-14T21:36:42Z
dc.language.rfc3066en
thesis.degree.disciplinebiochemistryen_NZ
thesis.degree.nameMaster of Scienceen_NZ
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.openaccessOpen
 Find in your library

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record