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dc.contributor.advisorBunn, Stephen James
dc.contributor.authorCarman, Fiona Helen
dc.date.available2012-07-31T21:54:34Z
dc.date.copyright2012
dc.identifier.citationCarman, F. H. (2012). Interleukin-6 Mediated Signalling in Adrenal Medullary Chromaffin Cells (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/2401en
dc.identifier.urihttp://hdl.handle.net/10523/2401
dc.description.abstractThe secretion of catecholamines and neuropeptides by the adrenal medulla plays a central role in the physiological adaptation to stress. Its activity is regulated by multiple factors including neuronal, hormonal and paracrine signals. Recent evidence suggests that the adrenal medulla may also be responsive to immune-derived signals such as cytokines. Such an interaction may form part of a wider bi-directional relationship believed to exist between the immune and neuroendocrine systems, disturbance of which may contribute to a number of stress-related pathologies. This study has employed isolated bovine adrenal medullary chromaffin cells together with immunocytochemistry and Western blotting procedures to demonstrate the pro-inflammatory cytokine interleukin-6 (IL-6) has a direct action on these neuroendocrine cells. Incubation with IL-6 caused a transient increase in the phosphorylation and thus activation of pERK1/2. This activation may have implications for the regulation of tyrosine hydroxylase activity and thus catecholamine synthesis. In addition to ERK activation IL-6 stimulated the tyrosine phosphorylation and nuclear localisation of signal transducer and activator of transcription-3 (STAT3) in approximately 70% of the cells, suggesting the cytokine may regulate chromaffin cell gene transcription. IL-6 also increased the serine phosphorylation of STAT3, probably through an ERK1/2 and Ca2+-influx dependent pathway. The biological significance of serine phosphorylation of STAT3 is unresolved. These data provide evidence that the neuroendocrine chromaffin cells are sensitive to IL-6, suggesting a pathway linking immune-derived signals to the adrenal medullary stress response. Importantly this IL-6 response has the potential to regulate both acute enzyme activity and gene expression, with potentially important implications for the homeostatic regulation of the inflammatory response.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectInterleukin-6
dc.subjectChromaffin cell
dc.subjectSTAT3
dc.subjectERK1/2
dc.titleInterleukin-6 Mediated Signalling in Adrenal Medullary Chromaffin Cells
dc.typeThesis
dc.date.updated2012-07-31T21:24:29Z
dc.language.rfc3066en
thesis.degree.disciplineAnatomy
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanno
otago.openaccessAbstract Only
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