Show simple item record

dc.contributor.advisorDearden, Peter
dc.contributor.advisorRaubenheimer, David
dc.contributor.authorMorgan, Sarah Margaret
dc.date.available2012-11-08T22:55:39Z
dc.date.copyright2012
dc.identifier.citationMorgan, S. M. (2012). Nutrition Forced Extension of Lifespan in Drosophila: A Whole-Genome Investigation (Thesis, Doctor of Philosophy). University of Otago. Retrieved from http://hdl.handle.net/10523/2579en
dc.identifier.urihttp://hdl.handle.net/10523/2579
dc.description.abstractThe life history trade off between longevity and reproduction is seen in a variety of animals. In the model organism Drosophila melanogaster, regulation of lifespan and reproductive rate are linked to various ratios of protein to carbohydrate diet ingested by the flies throughout their lifetime. Dietary Restriction and its effects on lifespan has been studied for many years, with consistent results seen between different organisms. Restriction of caloric intake is known to extend lifespan in different organisms. In Drosophila isocaloric diets of yeast (as a protein source) and sugar (as carbohydrate), in varying ratios, results in organisms trading-off lifespan and reproduction, implying macronutrient status rather than caloric restriction extends lifespan. The aim of the current study is to determine the patterns of gene expression associated with longevity in fruit fly. Drosophila were fed varying ratios of a protein/carbohydrate diet, and whole fly RNA was microarray assayed to identify genes differentially expressed between flies fed high carbohydrates and those fed high protein diets. Differentially expressed genes were verified with Q RT-PCR and a selection of candidate genes were analysed using P element interrupted mutant lines. Immune system stress assays were used to further investigate the response of flies raised on the specified diets. We show genes annotated as being involved in the immune system and stress response pathways to be significantly differentially expressed in array studies and overrepresented in gene ontology analysis; implying a role in the establishment and control of the lifespan extension phenomenon. We show an absence of significant differential expression in genes previously recorded as being involved in the control of the lifespan extension phenotype, and establish survivorship and lifespan data for a cohort of mutant and control lines. The current experiment provides direction for new functional assays, and adds to the growing body of knowledge as to what drives the extension of lifespan following dietary restriction.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectLongevity
dc.subjectNutrition
dc.subjectDietary Restriction
dc.subjectDrosophila melanogaster
dc.subjectStress Response
dc.subjectImmunity
dc.titleNutrition Forced Extension of Lifespan in Drosophila: A Whole-Genome Investigation
dc.typeThesis
dc.date.updated2012-11-08T21:55:49Z
dc.language.rfc3066en
thesis.degree.disciplineBiochemistry
thesis.degree.nameDoctor of Philosophy
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.interloanyes
otago.openaccessAbstract Only
 Find in your library

Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item is not available in full-text via OUR Archive.

If you would like to read this item, please apply for an inter-library loan from the University of Otago via your local library.

If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.

This item appears in the following Collection(s)

Show simple item record