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dc.contributor.advisorEaton-Rye, Julian John
dc.contributor.authorWaack, Max-Julian
dc.date.available2012-11-26T03:45:31Z
dc.date.copyright2012
dc.identifier.citationWaack, M.-J. (2012). Development of a Joliot-electrode for the detection of photosynthetically produced oxygen in cyanobacterial cells (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/2649en
dc.identifier.urihttp://hdl.handle.net/10523/2649
dc.description.abstractThe instrument I constructed in this project was a bare platinum electrode based on the original electrode by Pierre Joliot (Joliot P. (1972). Methods in Enzymology 24, 123–124) and the design by Johannes Messinger (Messinger J. (1993). Ph.D. thesis, Technische Universität Berlin). This electrode has the ability to detect the changes of oxygen tension with a high temporal resolution, enabling the exact measurement of the oxygen output from different photosynthetically active samples, such as thylakoid membranes and cyanobacterial cells. The sample was illuminated by an array of LEDs. The instrument also has the ability to keep the sample at a constant temperature, to reduce temperature related effects. A 3D-model and blueprints of the instrument were created and modified as necessary, before and after it was built. Special materials were acquired and the parts were manufactured in two workshops. New circuit diagrams for the detection circuit were planned based on original diagrams. A separate trigger circuit was also planned and built. Different analog-to-digital converters were acquired and tested. Corresponding programs for the handling, display and storage of data were programmed. The instrument was tested for basic functionality and flaws were corrected. Further tests were performed with different types of samples. As errors occurred they were eliminated or circumvented and the performance of the instrument was steadily improved. Several limitations with the current design were identified and additional improvements are recommended to enhance the utility of the electrode for determination of oxygen evolution by cyanobacterial cells.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectJoliot
dc.subjectJoliot-electrode
dc.subjectelectrode
dc.subjectbacteria
dc.subjectcyanobacteria
dc.subjectself build
dc.subjecttemperature
dc.subjectLED
dc.subjecttemporal resolution
dc.subjectthylakoids
dc.titleDevelopment of a Joliot-electrode for the detection of photosynthetically produced oxygen in cyanobacterial cells
dc.typeThesis
dc.date.updated2012-11-24T12:32:11Z
dc.language.rfc3066en
thesis.degree.disciplineBiochemistry
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanno
otago.openaccessAbstract Only
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