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dc.contributor.advisorWilbanks, Sigurd
dc.contributor.authorTuohy, Simon
dc.date.available2010-04-12T21:28:36Z
dc.date.copyright2009
dc.identifier.citationTuohy, S. (2009). Trigger Factor and its involvement in the repair of Photosystem II (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/317en
dc.identifier.urihttp://hdl.handle.net/10523/317
dc.description.abstractReplacement of the photosynthetic D1 protein is essential for continuation of photosynthesis and the life of a plant or cyanobacteria. Trigger Factor (TF) is an approximately 52 kDa molecular chaperone which may bind the ribosome-D1 complex prior to the association with the thylakoid targeted Signal Recognition Particle (cpSRP54). Trigger Factor binds to nearly all ribosomes, hence it is feasible that TF binds D1 protein and stalls until cpSRP54 can target it to SecY. The aim of this research was to create a TFSpec mutant of Synechocystis sp. PCC 6803 which would allow the characterisation of the TFSpec phenotype and to characterise the Synechocystis sp. PCC 6803 TF protein. The TFSpec strain showed several physiological differences from Wild Type (WT). The rate of O2 evolution by the TFSpec strain was slower than for the WT strain over the same period, and the growth rates of the TFSpec strain was lower than the WT strain. Trigger Factor was also differentially detected in various cellular fractions when pelleted with ribosomes purified from cells grown under different light con- ditions. Biophysical analysis of TF was done by Circular Dichroism and Dynamic Light Scattering to determine the structural similarity with TF from other species. Crystallisation of TF was also attempted. Implications of this research are discussed.en_NZ
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isoenen_NZ
dc.publisherUniversity of Otago
dc.rightshttp://www.otago.ac.nz/administration/policies/otago003228.htmlen_NZ
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.rights.urihttp://www.otago.ac.nz/administration/policies/otago003228.html
dc.subjectTrigger Factoren_NZ
dc.subjectphotosystemen_NZ
dc.titleTrigger Factor and its involvement in the repair of Photosystem IIen_NZ
dc.typeThesis
thesis.degree.disciplineBiochemistryen_NZ
thesis.degree.nameMaster of Scienceen_NZ
thesis.degree.grantorUniversity of Otagoen_NZ
thesis.degree.levelMasters Theses
otago.openaccessOpen
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