Biochemistry collection
Recent Deposits
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A Probe for Conformational Change of Hsc70 in Mammalian Cells under Stress Conditions
Hsc70 is a constitutively expressed member of the heat shock protein family. It has an important role in proteostasis and can stabilise nonnative proteins before being further matured in refolding, disaggregation, and ... -
Characterising TRIB1 Nanobodies using a Yeast Surface Display Platform with Flow Cytometry
The mammalian tribble (TRIB) pseudokinase family has three homologues, which act as an adaptor protein to regulate cell signalling pathways. TRIB proteins can bind the E3 ligase, Constitutive Photomorphogenesis Protein 1 ... -
Investigating RNF43 Mutations as Biomarkers for ctDNA Surveillance of Colorectal Cancer
This project aimed to investigate the utility of RNF43 mutations for circulating tumour DNA (ctDNA) surveillance of colorectal cancer (CRC). CRC is the second deadliest cancer in New Zealand. CRC patients currently under-go ... -
Identifying genes underlying adaptive traits of bulbing and bolting in onion (Allium cepa L.)
Onion (Allium cepa L.) is a biennial plant and completes its life cycle in two growing seasons i.e., forms bulbs in the first year and flowering and seed production occurs in the second year. The poor adaptation of onion ... -
Using hybridization chain reaction to investigate the homology of gene regulatory networks
How gene regulatory networks (GRNs) help produce phenotypic variation is a central question in developmental biology. A particularly well-studied class of GRNs are those performing segmentation: the subdivision of a ... -
Evolving eusociality: Honeybee queen pheromone induces a starvation response in a non-eusocial insect
Eusociality is a social structure characterised by the reproductive division of labour. A reproductively dominant female or females carry out reproduction, and her subordinate female workers have their reproduction ... -
Structural and functional characterisation of glutamate racemase isoform 2 from Acinetobacter baumannii
Acinetobacter baumannii is an opportunistic pathogen associated with high patient mortality within the Intensive Care Unit. The spread of antimicrobial resistance has resulted in some strains carrying up to 45 resistance ... -
The effects of large deletions on beta-lactam resistance in Pseudomonas aeruginosa
Background: P. aeruginosa is an opportunistic pathogen that presents a significant threat to immunocompromised individuals, particularly to those with cystic fibrosis (CF). Chronic lung infection is a major cause of ... -
The immunomodulatory protein, GIF, encoded by orf virus
Inflammatory diseases cause considerable morbidity throughout the world. Examples of inflammatory diseases include rheumatoid arthritis, Crohn’s disease, atherosclerosis and asthma. Current treatments options are insufficient, ... -
Regulation of Ark2C ubiquitylation activity
Ubiquitylation is a post-translational modification that regulates almost all signalling pathways in mammalian cells and its disruption is associated with a range of diseases. Ubiquitylation results in attachment of a small ... -
Evolution of ciprofloxacin resistance in Pseudomonas aeruginosa
Pseudomonas aeruginosa causes a wide range of infections. Ciprofloxacin is one of the most widely used antibiotics for the treatment of these infections. Antibiotic resistance in P. aeruginosa has made its eradication ... -
Annotation and evolution of bacterial ncRNA genes
Successful pathogenic bacteria must alter gene expression in response to changing and hostile environments. Non-coding RNAs (ncRNAs) contribute to adaptability and pathogenicity by forming complex regulatory networks, and ... -
The Effect of rs77275268 on Gene Expression at the Transcriptional Hub at 6q25.1
Breast cancer is a prevalent and harmful disease that is divided into subtypes based on tumour characteristics. The main subtype is oestrogen receptor positive (ER+), accounting for approximately 80% of all breast carcinomas. ... -
An Epigenetic Analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease affecting approximately 20,000 New Zealanders. Patients experience lifelong persistence of the disease with symptoms including a ... -
Single Cell Transcriptome Analysis in Prostate Cancer
Background: Single cell transcriptome studies have recently advanced to the analysis of millions of cells in a single pipeline. This type of analysis requires expensive, high maintenance platforms, precluding its use in ... -
Multiplex ddPCR for the detection of tumour-specific mutations in ctDNA
Circulating tumour DNA (ctDNA) are short extracellular DNA fragments that have been explored by a number of studies to potentially reflect a more complete representation of tumour burden without utilizing the highly invasive ... -
Investigating a putative neuroprotective pathway in Alzheimer’s disease.
Alzheimer’s disease is a debilitating neurological disorder, the worldwide cost of which has recently entered into the trillions of dollars ($USD) per annum. The primary cause of Alzheimer’s disease is believed to be the ... -
Targeted mutagenesis to investigate the role of low-molecular-weight proteins and a bicarbonate cofactor on the assembly and function of Photosystem II
Photosystem II (PS II) catalyses the oxidation of water at ambient temperatures and the formation of plastoquinol using light. This multisubunit complex functions as a dimer, each monomer is surrounded by 13 low-molecular-weight ... -
Candidate Compounds for the Chemoprevention of Hereditary Diffuse Gastric Cancer
Hereditary diffuse gastric cancer (HDGC) is a cancer syndrome caused by germline mutations in the tumour suppressor gene CDH1, which encodes the cell-cell adhesion protein E-cadherin. Mutations in CDH1 lead to increased ... -
uORF-mediated translational regulation of drought stress response gene MASP1 in Arabidopsis thaliana
Human-induced climate variability can subject global regions to water scarcity, prompting widespread crop failure. This calls for innovations that preserve global food security that do not further contribute to climate ...