Biochemistry collection
Recent Deposits
-
The role of Numb in honeybee ovary activation
Environmental signals in utero are throught to induce plastic changes to metabolism and gene regulation, causing diseases such as Type 2 Diabetes. For example, fetal undernutrition is associated with obesity, hyperglycemia, ... -
Analysing components of the Torso-activation module: Do they pattern the end-terminal regions of two Hymenopteran insect species, Apis mellifera and Nasonia vitripennis?
This project sought to determine if components of the Torso-activation module pattern the embryonic anterior and posterior ends of the honey bee, Apis mellifera, and the parasitoid wasp, Nasonia vitripennis via a patterning ... -
The effects of 5-fluorocytosine on Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic pathogen responsible for a variety of infections. P. aeruginosa presents a challenge to conventional antibiotic therapeutics because of the emergence and rapid spread of antibiotic ... -
Genomic Evolution and Iron Uptake in Pseudomonas aeruginosa
Recombination and lateral transfer of genomic material between bacterial strains have long been recognised as important to the evolution of a species. Recent developments in sequencing technologies has resulted in a flood ... -
Novel methods for influenza drug research and development: Cloning, expression and purification of influenza neuraminidase for inhibitory assays
Influenza is a highly contagious virus which can cause mild to severe disease. The most effective drugs for treating influenza, are the neuraminidase inhibitors (NAIs), but resistance to these inhibitors has emerged. New ... -
Selection and Metabolic Disease in the Pacific
An example of a "signature of selection" in a population is a region of the genome that exhibits a reduction in genetic variability with a particular linkage disequilibrium pattern. This reduction in variation can arise ... -
Bulbing and flowering regulation in bulb onion (Allium cepa L.)
Bulb onion is an important vegetable crop cultivated worldwide for salad and culinary purposes. Onions have a biennial life cycle; during the first year the bulb is formed followed by flowering in the second year. The bulb ... -
SLC2A9 and Hyperuricemia: A Locus-Wide Association Study To Identify Population-Specific Genetic Variants In New Zealand Māori and Pacific People
Hyperuricemia, pathologically defined as the presence of an elevated level of serum urate, is a prerequisite for gouty arthritis. The solute carrier family 2 member 9 (SLC2A9) gene that encodes a urate transporter tops the ... -
Investigation into the transcriptional regulation of DUO1: determining the fate of the male germline in flowering plants
The pollen grain is responsible for the production and delivery of sperm cells to the female gametes in flowering plants. Early in pollen development, there is an asymmetric division that is critical for the specification ... -
Comparison of FRET probes of conformational changes in DnaK and Hsc70
Hsp70s are one of the most well conserved protein families known, yet little of their in vivo activity is understood and experimental resources required to investigate their activity. All Hsp70s are made up of two highly ... -
The role of the SVP gene family in controlling bud dormancy
SHORT VEGETATIVE PHASE (SVP)-like genes in the StMADS11 gene family have roles in the regulation of flowering time in annual plants and there is evidence that they also function in the regulation of dormancy in perennials. ... -
Mining bioactive peptides from sheep cheese whey β-lactoglobulin using food grade proteases
Sheep cheese whey (SCW) is a by-product of the cheese making process that is often considered waste and treated as an environmental pollutant. This research project stemmed from a recent change in view that rather than ... -
Roles of Introns
Many genes of eukaryotes and viruses contain introns. The roles of introns are not fully understood because many of them seem dispensable. To address this long-standing question, comparative genomics and ‘multi-omic’ ... -
Chemotaxis and inhibition of the kauri killer, Phytophthora agathidicida
Phytophthora are devastating pathogens which cause diseases in thousands of economically and ecologically important plants worldwide. Often referred to as 'fungus-like', Phytophthora are a genus of eukaryotic oomycetes. ... -
Introducing CRISPR/Cas9 into the kiwifruit pathogen Pseudomonas syringae pv. actinidiae (Psa)
Pseudomonas syringae pv. actinidiae (Psa) is an emerging bacterial pathogen of kiwifruit, a major primary export of New Zealand. The emergence of Psa in New Zealand in 2010 caused immense financial grief with the disease ... -
Investigating the regulation of Arkadia activity in cells
Ubiquitylation is a post translational modification the regulates a wide variety of cellular processes. The attachment of ubiquitin onto a substrate requires the activity of three enzymes; E1, E2 and E3. As ubiquitylation ... -
Association of Polynesian-specific variants of PRPSAP1 and SLC22A3 genes with gout.
Gout is a complex disease caused by over-production and/or under-excretion of urate, which causes hyperuricaemia. This leads to urate deposition, and initiates the formation of monosodium urate (MSU) crystals in joints and ... -
Characterisation of Plant Cysteine Dioxygenase
Many organisms possess a non-heme, mononuclear iron enzyme termed cysteine dioxygenase (CDO) that aids in homeostasis. This enzyme irreversibly adds molecular oxygen to the thiol group of the N-terminal cysteine residue ... -
Engineering quorum-quenching enzymes
Biofilms are structured communities of bacteria that adhere to surfaces and shield bacteria from hostile surroundings. They are implicated in 75% of all infections in humans and are difficult to treat. New therapies are ... -
Ice activities in New Zealand Chionochloa species
Overwintering plants produce ice active agents with low thermal hysteresis but a higher ability to inhibit the recrystallization of ice crystals already formed. Many plants also minimize the damage from freezing by triggering ...