Biochemistry collection
Recent Deposits
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Biochemistry and Biophysics of the ASK1-Prdx1 Axis
Peroxiredoxin 1 (Prdx1) is an abundant cytoplasmic human thiol peroxidase. It functions to detoxify reactive oxygen species, in particular, hydrogen peroxide (H2O2). The canonical mechanism of Prdx1 activity involves the ... -
The Establishment and Characterisation of Gastric Organoids as a Model for Hereditary Diffuse Gastric Cancer
Cancer is an exceptionally complex disease which requires an appropriately sophisticated model to facilitate its research. Organoids are 3D structures generated from stem cells, which recapitulate a target organ. These ... -
The role of molecular genetic variation and population structure in the genetic improvement of the honeybee, Apis mellifera
Animal breeding has evolved into a highly sophisticated field, making substantial changes to numerous livestock species worldwide. Such changes are largely absent in the Western Honeybee, Apis mellifera. Direct transfer ... -
The Epigenetics of Colorectal Cancer
Aberrant DNA methylation patterns are a universal feature of cancer, yet we understand relatively little of the cause and consequence of DNA methylation in the cancer process. Colorectal cancer (CRC) morbidity and mortality ... -
Biophysical characterisation of the bacterial chemosensory protein, PscF
In bacteria, movement in response to a chemical stimulus is known as chemotaxis. Chemotaxis is controlled by a class of ligand binding proteins called chemoreceptors and works to bias cell movement towards environments ... -
Flowering time control in legumes: investigation of the photoperiod pathway
Understanding how plants integrate environmental and endogenous cues to ensure flowering occurs at a time of highest reproductive success is of significant interest. Flowering time is a key trait in agriculture as flowering ... -
RNF125 activated ubiquitin transfer by Ube2K
The covalent attachment of ubiquitin to substrate proteins (ubiquitylation) is a post-translational modification involved in numerous cell signalling pathways, most notably the process of protein degradation. Ubiquitylation ... -
Biochemistry of Apoptosis Signal-regulating Kinase 1 Regulation and Hydrogen Peroxide Signalling
All cells within an organism must respond to their environment. Responses are regulated by intracellular signalling pathways and signalling molecules. One such molecule is hydrogen peroxide (H2O2). H2O2 is required in many ... -
The role of PsbX and PsbY in Photosystem II of Synechocystis sp. PCC 6803
Photosystem II (PS II) is the water-plastoquinone oxido-reductase responsible for splitting water in photosynthesis. The core of the enzyme is surrounded by 13 low-molecular-weight (LMW) proteins (<10 kDa). In this study, ... -
The role of Numb in honeybee ovary activation
Environmental signals in utero are throught to induce plastic changes to metabolism and gene regulation, causing diseases such as Type 2 Diabetes. For example, fetal undernutrition is associated with obesity, hyperglycemia, ... -
Analysing components of the Torso-activation module: Do they pattern the end-terminal regions of two Hymenopteran insect species, Apis mellifera and Nasonia vitripennis?
This project sought to determine if components of the Torso-activation module pattern the embryonic anterior and posterior ends of the honey bee, Apis mellifera, and the parasitoid wasp, Nasonia vitripennis via a patterning ... -
The effects of 5-fluorocytosine on Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic pathogen responsible for a variety of infections. P. aeruginosa presents a challenge to conventional antibiotic therapeutics because of the emergence and rapid spread of antibiotic ... -
Genomic Evolution and Iron Uptake in Pseudomonas aeruginosa
Recombination and lateral transfer of genomic material between bacterial strains have long been recognised as important to the evolution of a species. Recent developments in sequencing technologies has resulted in a flood ... -
Novel methods for influenza drug research and development: Cloning, expression and purification of influenza neuraminidase for inhibitory assays
Influenza is a highly contagious virus which can cause mild to severe disease. The most effective drugs for treating influenza, are the neuraminidase inhibitors (NAIs), but resistance to these inhibitors has emerged. New ... -
Selection and Metabolic Disease in the Pacific
An example of a "signature of selection" in a population is a region of the genome that exhibits a reduction in genetic variability with a particular linkage disequilibrium pattern. This reduction in variation can arise ... -
Bulbing and flowering regulation in bulb onion (Allium cepa L.)
Bulb onion is an important vegetable crop cultivated worldwide for salad and culinary purposes. Onions have a biennial life cycle; during the first year the bulb is formed followed by flowering in the second year. The bulb ... -
SLC2A9 and Hyperuricemia: A Locus-Wide Association Study To Identify Population-Specific Genetic Variants In New Zealand Māori and Pacific People
Hyperuricemia, pathologically defined as the presence of an elevated level of serum urate, is a prerequisite for gouty arthritis. The solute carrier family 2 member 9 (SLC2A9) gene that encodes a urate transporter tops the ... -
Investigation into the transcriptional regulation of DUO1: determining the fate of the male germline in flowering plants
The pollen grain is responsible for the production and delivery of sperm cells to the female gametes in flowering plants. Early in pollen development, there is an asymmetric division that is critical for the specification ... -
Comparison of FRET probes of conformational changes in DnaK and Hsc70
Hsp70s are one of the most well conserved protein families known, yet little of their in vivo activity is understood and experimental resources required to investigate their activity. All Hsp70s are made up of two highly ... -
The role of the SVP gene family in controlling bud dormancy
SHORT VEGETATIVE PHASE (SVP)-like genes in the StMADS11 gene family have roles in the regulation of flowering time in annual plants and there is evidence that they also function in the regulation of dormancy in perennials. ...