Biochemistry collection
Recent Deposits
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Characterisation of conserved amino acids from the low-oxygen expressed D1 protein in the model organism Synechocystis sp. PCC 6803
Oxygenic photosynthesis exploits light, water and carbon dioxide to transform light energy into chemical energy. This process is fundamental in shaping the current oxygenic atmosphere and supports most life on earth. The ... -
Investigating the roles of specific amino acid substitutions found in the D1' and far-red forms of the Photosystem II reaction centre D1 protein
Photosystem II is a highly conserved membrane-protein complex that plays an integral role in oxygenic photosynthesis as a light-driven water-plastoquinone oxidoreductase. One of the most important proteins within Photosystem ... -
Exploring hot spot mutagenesis as a means of dimer disruption in glutamate racemase from Mycobacterium smegmatis
The emergence of multidrug-resistant Mycobacterium tuberculosis strains has highlighted the need to develop new drugs for the treatment of tuberculosis. Glutamate racemase (MurI) has been identified as an essential enzyme ... -
The Application of Circulating Tumour DNA to the Management of Gastrointestinal Cancers.
Circulating tumour DNA (ctDNA) are small fragments of DNA released by tumours into the circulation. These fragments contain pathogenic mutations which can be detected and quantified by various next generation sequencing ... -
Genetics of gout - Progression from hyperuricaemia to gout
Gout is an inflammatory arthritis that is prevalent in New Zealand (NZ) populations, with a higher frequency in Māori (6.06%) and Pacific Islanders (7.60%) in comparison to NZ Europeans (3.24%). This inflammatory arthritis ... -
An Inter-Species Investigation into the Role of Cytochrome c in Apoptosis
Cytochrome c is a protein that has important roles in electron transport and cell death (apoptosis). In its role during the intrinsic apoptosis pathway, cytochrome c is released from the mitochondria for it to interact ... -
Low-input transcriptomics of developing pollen in Arabidopsis thaliana
Pollen is the male gametophyte of flowering plants that deliver sperm cells to the female gametophyte during fertilization. Pollen development begins with the reduction division (meiosis) of a single pollen mother cell to ... -
A Probe for Conformational Change of Hsc70 in Mammalian Cells under Stress Conditions
Hsc70 is a constitutively expressed member of the heat shock protein family. It has an important role in proteostasis and can stabilise nonnative proteins before being further matured in refolding, disaggregation, and ... -
Characterising TRIB1 Nanobodies using a Yeast Surface Display Platform with Flow Cytometry
The mammalian tribble (TRIB) pseudokinase family has three homologues, which act as an adaptor protein to regulate cell signalling pathways. TRIB proteins can bind the E3 ligase, Constitutive Photomorphogenesis Protein 1 ... -
Investigating RNF43 Mutations as Biomarkers for ctDNA Surveillance of Colorectal Cancer
This project aimed to investigate the utility of RNF43 mutations for circulating tumour DNA (ctDNA) surveillance of colorectal cancer (CRC). CRC is the second deadliest cancer in New Zealand. CRC patients currently under-go ... -
Identifying genes underlying adaptive traits of bulbing and bolting in onion (Allium cepa L.)
Onion (Allium cepa L.) is a biennial plant and completes its life cycle in two growing seasons i.e., forms bulbs in the first year and flowering and seed production occurs in the second year. The poor adaptation of onion ... -
Using hybridization chain reaction to investigate the homology of gene regulatory networks
How gene regulatory networks (GRNs) help produce phenotypic variation is a central question in developmental biology. A particularly well-studied class of GRNs are those performing segmentation: the subdivision of a ... -
Evolving eusociality: Honeybee queen pheromone induces a starvation response in a non-eusocial insect
Eusociality is a social structure characterised by the reproductive division of labour. A reproductively dominant female or females carry out reproduction, and her subordinate female workers have their reproduction ... -
Structural and functional characterisation of glutamate racemase isoform 2 from Acinetobacter baumannii
Acinetobacter baumannii is an opportunistic pathogen associated with high patient mortality within the Intensive Care Unit. The spread of antimicrobial resistance has resulted in some strains carrying up to 45 resistance ... -
The effects of large deletions on beta-lactam resistance in Pseudomonas aeruginosa
Background: P. aeruginosa is an opportunistic pathogen that presents a significant threat to immunocompromised individuals, particularly to those with cystic fibrosis (CF). Chronic lung infection is a major cause of ... -
The immunomodulatory protein, GIF, encoded by orf virus
Inflammatory diseases cause considerable morbidity throughout the world. Examples of inflammatory diseases include rheumatoid arthritis, Crohn’s disease, atherosclerosis and asthma. Current treatments options are insufficient, ... -
Regulation of Ark2C ubiquitylation activity
Ubiquitylation is a post-translational modification that regulates almost all signalling pathways in mammalian cells and its disruption is associated with a range of diseases. Ubiquitylation results in attachment of a small ... -
Evolution of ciprofloxacin resistance in Pseudomonas aeruginosa
Pseudomonas aeruginosa causes a wide range of infections. Ciprofloxacin is one of the most widely used antibiotics for the treatment of these infections. Antibiotic resistance in P. aeruginosa has made its eradication ... -
Annotation and evolution of bacterial ncRNA genes
Successful pathogenic bacteria must alter gene expression in response to changing and hostile environments. Non-coding RNAs (ncRNAs) contribute to adaptability and pathogenicity by forming complex regulatory networks, and ... -
The Effect of rs77275268 on Gene Expression at the Transcriptional Hub at 6q25.1
Breast cancer is a prevalent and harmful disease that is divided into subtypes based on tumour characteristics. The main subtype is oestrogen receptor positive (ER+), accounting for approximately 80% of all breast carcinomas. ...