Browsing Biochemistry collection by Date Published
Now showing items 1-20 of 222
-
Multiplex ddPCR for the detection of tumour-specific mutations in ctDNA
Circulating tumour DNA (ctDNA) are short extracellular DNA fragments that have been explored by a number of studies to potentially reflect a more complete representation of tumour burden without utilizing the highly invasive ... -
An Epigenetic Analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease affecting approximately 20,000 New Zealanders. Patients experience lifelong persistence of the disease with symptoms including a ... -
Single Cell Transcriptome Analysis in Prostate Cancer
Background: Single cell transcriptome studies have recently advanced to the analysis of millions of cells in a single pipeline. This type of analysis requires expensive, high maintenance platforms, precluding its use in ... -
The Effect of rs77275268 on Gene Expression at the Transcriptional Hub at 6q25.1
Breast cancer is a prevalent and harmful disease that is divided into subtypes based on tumour characteristics. The main subtype is oestrogen receptor positive (ER+), accounting for approximately 80% of all breast carcinomas. ... -
Annotation and evolution of bacterial ncRNA genes
Successful pathogenic bacteria must alter gene expression in response to changing and hostile environments. Non-coding RNAs (ncRNAs) contribute to adaptability and pathogenicity by forming complex regulatory networks, and ... -
Evolution of ciprofloxacin resistance in Pseudomonas aeruginosa
Pseudomonas aeruginosa causes a wide range of infections. Ciprofloxacin is one of the most widely used antibiotics for the treatment of these infections. Antibiotic resistance in P. aeruginosa has made its eradication ... -
Regulation of Ark2C ubiquitylation activity
Ubiquitylation is a post-translational modification that regulates almost all signalling pathways in mammalian cells and its disruption is associated with a range of diseases. Ubiquitylation results in attachment of a small ... -
The immunomodulatory protein, GIF, encoded by orf virus
Inflammatory diseases cause considerable morbidity throughout the world. Examples of inflammatory diseases include rheumatoid arthritis, Crohn’s disease, atherosclerosis and asthma. Current treatments options are insufficient, ... -
The effects of large deletions on beta-lactam resistance in Pseudomonas aeruginosa
Background: P. aeruginosa is an opportunistic pathogen that presents a significant threat to immunocompromised individuals, particularly to those with cystic fibrosis (CF). Chronic lung infection is a major cause of ... -
Structural and functional characterisation of glutamate racemase isoform 2 from Acinetobacter baumannii
Acinetobacter baumannii is an opportunistic pathogen associated with high patient mortality within the Intensive Care Unit. The spread of antimicrobial resistance has resulted in some strains carrying up to 45 resistance ... -
Characterisation of a new role for the UBA domain in ubiquitin chain synthesis by Ube2K
Ubiquitylation is a post-translational modification in which the protein Ubiquitin (Ub) is attached to other proteins, dictating their fate. Ubiquitylation requires the action of three groups of enzymes, Ub activating (E1) ... -
Using targeted mutagenesis to investigate the role of the bicarbonate ligated to the non-heme iron of Photosystem II
Photosystem II (PS II) is a photochemical oxidoreductase enzyme capable of splitting water and initiating photosynthesis. Photosystem II functions by removing electrons from water, and transferring them via a series of ... -
The Epigenetics of Colorectal Cancer
Aberrant DNA methylation patterns are a universal feature of cancer, yet we understand relatively little of the cause and consequence of DNA methylation in the cancer process. Colorectal cancer (CRC) morbidity and mortality ... -
The PvdF enzyme of Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic bacterium which requires iron for growth and survival. Under iron limiting conditions, P.aeruginosa secrete iron scavenging compounds called pyoverdines. Pyoverdines consist of a ... -
Exosome mRNA localisation: Directing mRNA localisation to exosomes
Exosomes are small vesicles containing proteins, miRNAs and mRNAs. Exosomes are secreted and taken up by neighbouring cells and the contents move into the recipient cell, where they are biologically active (e.g. the mRNA ... -
The role of molecular genetic variation and population structure in the genetic improvement of the honeybee, Apis mellifera
Animal breeding has evolved into a highly sophisticated field, making substantial changes to numerous livestock species worldwide. Such changes are largely absent in the Western Honeybee, Apis mellifera. Direct transfer ... -
Investigating a putative neuroprotective pathway in Alzheimer’s disease.
Alzheimer’s disease is a debilitating neurological disorder, the worldwide cost of which has recently entered into the trillions of dollars ($USD) per annum. The primary cause of Alzheimer’s disease is believed to be the ... -
Disrupting Pseudomonas aeruginosa quorum sensing
Many clinically relevant bacteria use a population-dependent communication network - known as quorum sensing - to regulate the processes essential for the establishment of infections. Quorum sensing enables pathogens to ... -
The Establishment and Characterisation of Gastric Organoids as a Model for Hereditary Diffuse Gastric Cancer
Cancer is an exceptionally complex disease which requires an appropriately sophisticated model to facilitate its research. Organoids are 3D structures generated from stem cells, which recapitulate a target organ. These ... -
Biochemistry and Biophysics of the ASK1-Prdx1 Axis
Peroxiredoxin 1 (Prdx1) is an abundant cytoplasmic human thiol peroxidase. It functions to detoxify reactive oxygen species, in particular, hydrogen peroxide (H2O2). The canonical mechanism of Prdx1 activity involves the ...