Browsing Biochemistry collection by Title
Now showing items 1-20 of 222
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2.8 Å Structure of an ATP-bound Hsp70 from Escherichia coli
Heat shock protein 70 (Hsp70) is an important molecular chaperone of the proteostasis network, mediating protein folding and stability. Malfunction of proteostasis contributes to diseases as diverse as cancer and Alzheimer’s. ... -
A Bioinformatics Approach to Synthetic Lethal Interactions in Cancer with Gene Expression Data
Introduction Synthetic lethal genetic interactions are re-emerging as an important concept in the post-genomics era due to their potential for use in precision medicine against cancers. Synthetic lethal drug design ... -
A FRET reporter of conformation in Hsc70
Hsp70s are the most well conserved protein family known, and yet little of their activity within the cell is understood. Hsc70 is a constitutively expressed member of the Hsp70 family found in the cytosol of almost all ... -
A Genomic Investigation of Waddington’s Assimilation
Lamarck believed that traits acquired during an organism’s lifetime could be passed onto the next generation. Although this idea of the inheritance of acquired characters was discarded due to lack of experimental evidence, ... -
A Metabolic Health Study of Pacific Adolescents Investigating the metabolic health of Pacific adolescents in New Zealand: Environmental and genetic risk factors.
Adults of Pacific descent residing in New Zealand have more than double the prevalence of diabetes, obesity, gout and the metabolic syndrome (MS), compared to the general population. Genetic, biochemical and environmental ... -
A probing tail of our molecular chaperones
Molecular chaperone proteins play a pivotal role in maintaining normal proteostasis within the cell by aiding the correct folding of nascent chains. Understanding how these proteins function, has implications for a milieu ... -
A rapid response to iron in human cells
Abstract Background: Given the importance of iron in human health, surprisingly there are many gaps in our knowledge of how cells can rapidly respond to changes in extracellular iron levels. There is a lack of research ... -
A search for synthetic lethality between Polo-like Kinase 3 and E- Cadherin
Hereditary diffuse gastric cancer (HDGC) is an autosomal dominant condition caused by a mutation in the tumour suppressor gene E-cadherin, (CDH1). It predisposes to a 70% likelihood of developing highly penetrant diffuse ... -
A Synthetic Lethal Approach to the Development of Novel Cancer Therapies
The cell-cell adhesion protein E-cadherin (CDH1) is a tumour suppressor that is required to maintain cell adhesion, cell polarity and cell survival signalling. Somatic mutations in CDH1 are common in diffuse gastric cancer ... -
An Epigenetic Analysis of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease affecting approximately 20,000 New Zealanders. Patients experience lifelong persistence of the disease with symptoms including a ... -
An interaction between miR-124 and FEZF2 in neuronal development
Forebrain embryonic zinc finger-like protein 2 (FEZF2) is a transcription factor involved in the embryonic development, differentiation and projection fates of neurons in the brain. It is expressed at high levels ... -
An Intracellular Probe of Hsc70:Substrate Interactions
Hsc70 is a constitutively active member of the heat shock chaperone family. It has many roles in all cell types, including prevention of protein aggregation, uncoating of clathrin vesicles, and involvement in the maturation ... -
An investigation into the potential use of the histone deacetylase inhibitor, valproic acid for the prevention of gastric cancer in high-risk families
Hereditary diffuse gastric cancer (HDGC) is a cancer syndrome caused by germline mutation of the CDH1 gene that encodes the cell adhesion tumor suppressor protein E-cadherin. Mutation carriers have a lifetime risk higher ... -
An investigation of carotenoids and a carotenoid-binding protein in sea urchin gonad colour production
The sea urchin Evechinus chloroticus, known as kina, is endemic to New Zealand and is harvested for its edible gonads. Kina are culturally important to Maori and are also commercially harvested to supply roe to domestic ... -
An investigation of the coelomic fluid and coelomocytes in a New Zealand sea urchin (Kina) Evechinus chloroticus
Abstract Sea urchins fulfil a vital role as a simple scientific model organism and as such have remained a well-used tool in the study of embryonic development and immunology. Sea urchins are known to be immunologically ... -
Analysing components of the Torso-activation module: Do they pattern the end-terminal regions of two Hymenopteran insect species, Apis mellifera and Nasonia vitripennis?
This project sought to determine if components of the Torso-activation module pattern the embryonic anterior and posterior ends of the honey bee, Apis mellifera, and the parasitoid wasp, Nasonia vitripennis via a patterning ... -
Analysis of fungal inteins
Homing endonucleases are highly specific DNases, commonly called HEGs, encoded by self-splicing intein and intron sequences. They recognise long target sequences and confer mobility to their host sequence by introducing a ... -
Analysis of the Aspergillus nidulans PRP8 intein
Inteins are naturally-occurring intervening sequences that are translated along with their host protein. The intein insertion is excised from its host protein post-translationally in an autocatalytic process known as intein ... -
Annotation and evolution of bacterial ncRNA genes
Successful pathogenic bacteria must alter gene expression in response to changing and hostile environments. Non-coding RNAs (ncRNAs) contribute to adaptability and pathogenicity by forming complex regulatory networks, and ... -
Approaches to identify candidate genes for resistance to facial eczema disease in sheep
Facial eczema disease (FE) is a secondary photosensitisation disease of ruminants caused by exposure to the mycotoxin sporidesmin. Resistance to FE has a significant genetic component and previous research has included a ...