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dc.contributor.advisorEaton-Rye, Julian
dc.contributor.authorLamb, Jacob Joseph
dc.date.available2013-01-21T22:31:24Z
dc.date.copyright2013
dc.identifier.citationLamb, J. J. (2013). The Role of Type IV Pili of Synechocystis sp. PCC 6803 in Extracellular Electron Transfer (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/3683en
dc.identifier.urihttp://hdl.handle.net/10523/3683
dc.description.abstractThe objective of this research was to determine whether the Type IV pili of Synechocystis sp. PCC 6803 are involved in electron donation to the extracellular environment. In conjunction with the current theories and published observations from other research groups, the findings presented in this research are suggestive of interplay between both the photosynthetic and respiratory electron transport chains and the pili. Gene deletion studies also advocate possible cytochrome intermediates interacting within the electron transport chain of both the photosynthetic and respiratory complexes, involved in donating electrons to the pili. The construction and implementation of four novel instruments has been outlined in this project, consisting of: (1) a small battery powered spectrophotometer capable of measuring turbidity of liquid culture, (2) solid-grown-culture density plate-reader, (3) chlorophyll concentration fluorometer and (4) an apparatus that measures voltage indicative of electron donation to an iron electrode. The physiological characterisation component of this project explored the function of the Type IV pili in Synechocystis sp. PCC 6803 in facilitating electron donation to extracellular acceptors. A link has been observed between oxidized iron minerals and the presence of these pili and cytochromes suggesting that they may provide the organism with a utility for sensing iron scarcity. An additional phenotype implies a function of these pili in protecting against oxidative stress by dissipation of excess excitation energy. Together these observations indicate the potential of electron donation by Type IV in Synechocystis sp. 6803, which may be exploited in the future to convert solar energy into electricity.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectphotosynthesis
dc.subjectbiotechnology
dc.subjectbiochemistry
dc.subjectsustainable energy
dc.subjectbioenergy
dc.subjectsynechocystis
dc.subjectcyanobacteria
dc.subjectelectron donation
dc.titleThe Role of Type IV Pili of Synechocystis sp. PCC 6803 in Extracellular Electron Transfer
dc.typeThesis
dc.date.updated2013-01-21T21:23:34Z
dc.language.rfc3066en
thesis.degree.disciplineBiochemistry
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanno
otago.openaccessAbstract Only
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