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dc.contributor.advisorMarshall, Craig
dc.contributor.advisorPeake, Barrie
dc.contributor.authorPerez-Diaz, Noelia
dc.date.available2013-09-23T20:35:11Z
dc.date.copyright2013
dc.identifier.citationPerez-Diaz, N. (2013). Biochemical aspects of cadmium binding to oysters (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/4300en
dc.identifier.urihttp://hdl.handle.net/10523/4300
dc.description.abstractIt has been found in some places around New Zealand, specifically in Tasman Bay, Foveaux Strait and Chatham islands, that the concentration of cadmium (Cd) in the Bluff oyster (Ostrea chilensis) is very high, and similar to that of oysters from polluted areas. This is unexpected because both water and sediments are very clean in these places, and the environmental concentration of Cd is very low. Cadmium is a hazardous heavy metal classified by the World Health Organisation within the group 1 (Human Carcinogens) and listed by the Comprehensive Environmental Response, Compensation, and Liability Act as being No7 in its list of hazardous materials. However, epidemiological studies undertaken in people consuming Bluff oysters proved no sign of Cd-intoxication which could imply that the cadmium is not free in the oyster, but protein bound. At present, it is unknown which protein might be responsible for the binding of Cd in the Bluff oyster. This study aimed to develop a procedure to localize and identify the Cd-binding protein (Cd-BP) in the Bluff oyster. A combination of gel filtration chromatography (GFC) to separate the proteins of the Bluff oyster, and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to analyse the metal content of the fractions, proved to be the most reliable procedure and resulted in the isolation of one candidate Cd-BP. Further analysis using MALDI MS/MS and a de novo sequencing of the candidate identified the hypothetical protein PPAM2_20180 (Pseudomonas sp.). The present study has also contributed to the data available on the distribution of selected trace metals between the soluble and insoluble fractions in oyster tissue, and made a new contribution to the literature by showing the distribution of metals among the high molecular weight proteins (30 – 600 kDa) of the soluble fraction of the Bluff oyster tissue.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectNew
dc.subjectZealand
dc.subjectCadmium
dc.subjectOyster
dc.titleBiochemical aspects of cadmium binding to oysters
dc.typeThesis
dc.date.updated2013-09-23T08:50:13Z
dc.language.rfc3066en
thesis.degree.disciplineBiochemistry
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.openaccessOpen
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