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dc.contributor.advisorCannon, Richard
dc.contributor.advisorNiimi, Kyoko
dc.contributor.authorNewsham-West, Kathryn J
dc.date.available2013-10-24T01:31:10Z
dc.date.copyright2013
dc.identifier.citationNewsham-West, K. J. (2013). Overcoming the antifungal drug resistance of biofilms on dental acrylic (Thesis, Doctor of Clinical Dentistry). University of Otago. Retrieved from http://hdl.handle.net/10523/4354en
dc.identifier.urihttp://hdl.handle.net/10523/4354
dc.description.abstractIntroduction RC21v3 is an octapeptide derivative that inhibits the drug efflux pump that confers azole resistance on the yeast Candida albicans -an opportunistic pathogen that can cause denture stomatitis. C. albicans often forms biofilms on oral surfaces including denture prostheses, and biofilm cells are more drug resistant than planktonic cells growing as a suspension in liquid medium. Aim The objective of this research was to determine whether RC21v3 acts synergistically with fluconazole (FLC) to inhibit the growth of C. albicans cells in planktonic cultures and during growth as a biofilm. Methods Minimum growth inhibition concentration (MIC) assays were undertaken to measure the inhibitory effect of FLC and RC21v3 on C. albicans cells growing as planktonic cultures. Checkerboard MIC assays were used to measure the effect of combinations of FLC with RC21v3 on both planktonic and biofilm growth. The inhibition of C. albicans biofilm growth on denture acrylic was measured using crystal violet staining of biofilm cells. The effect of FLC and/or RC21v3 on biofilm structure was investigated using confocal microscopy. Results Checkerboard MIC assays showed that RC21v3 acted synergistically with FLC to inhibit the planktonic growth of three C. albicans strains: ATCC 10261 and MML610 which are FLC-susceptible, and MML611 which is FLC-resistant. In addition, the combination of RC21v3 with FLC in growth assays resulted in FLC becoming fungicidal to all three strains. A novel dental acrylic biofilm model was developed. As it demonstrated reproducible growth of C. albicans, it could be used to measure biofilm growth inhibition. FLC inhibited the growth of C. albicans biofilms and checkerboard assays showed that RC21v3 acted synergistically to reduce the growth of ATCC10261 biofilms. Conclusion This is the first demonstration that RC21v3 can chemosensitize C. albicans biofilms to fluconazole, and so may be of use in treatment of patients with FLC-resistant biofilms on denture surfaces.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectCandida
dc.subjectalbicans
dc.subjectantifungal
dc.subjectresistance
dc.subjectbiofilm
dc.subjectdental
dc.titleOvercoming the antifungal drug resistance of biofilms on dental acrylic
dc.typeThesis
dc.date.updated2013-10-23T22:58:38Z
dc.language.rfc3066en
thesis.degree.disciplineOral rehabilitation
thesis.degree.nameDoctor of Clinical Dentistry
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.openaccessOpen
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