Gene transfer to restore vitamin C synthesis in human cell line
|dc.contributor.author||Flett, Teresa Joy|
|dc.identifier.citation||Flett, T. J. (2013). Gene transfer to restore vitamin C synthesis in human cell line (Thesis, Bachelor of Biomedical Sciences with Honours). University of Otago. Retrieved from http://hdl.handle.net/10523/4410||en|
|dc.description.abstract||Vitamin C (ascorbate) is deficient in humans due to the lack in the final step in the biosynthesis pathway, Gulonolactone oxidase (Gulo). Ascorbate has been studied as a possible cancer therapy since the 1970’s, however results have been inconclusive. One of the reasons for this is the variability in ascorbate transport to the tumour. This study aims to design a biological tool that allows the study of ascorbate in cancer without transport being a variable. HepG2 cells were transfected with pCMV6-‐Gulo and tested for genome incorporation by PCR, Gulo enzyme expression by western blot and functionality by HPLC-‐ECD. One successful clone was established and a level of ascorbate comparable to previous studies was observed when the Gulo substrate (gulonolactone) was added. Transfected cells were assayed for HIF-1α levels as a sign of tumour aggression. When ascorbate is added to the cells in 1% oxygen HIF-1α levels are reduced. Interestingly a similar level of reduction was also observed when gulonolactone is added to the cells|
|dc.publisher||University of Otago|
|dc.rights||All items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.|
|dc.subject||Hypoxia inducable factor 1|
|dc.title||Gene transfer to restore vitamin C synthesis in human cell line|
|thesis.degree.name||Bachelor of Biomedical Sciences with Honours|
|thesis.degree.grantor||University of Otago|
Files in this item
There are no files associated with this item.
This item is not available in full-text via OUR Archive.
If you would like to read this item, please apply for an inter-library loan from the University of Otago via your local library.
If you are the author of this item, please contact us if you wish to discuss making the full text publicly available.