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dc.contributor.advisorKemp, Roslyn
dc.contributor.authorDunn, Elliott Thomas James
dc.date.available2014-06-26T03:00:29Z
dc.date.copyright2014
dc.identifier.citationDunn, E. T. J. (2014). It all starts in the gut: The intestinal immune cell infiltrate in inflammatory bowel disease and ankylosing spondylitis (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/4867en
dc.identifier.urihttp://hdl.handle.net/10523/4867
dc.description.abstractInflammatory Bowel Disease (IBD) and Ankylosing Spondylitis (AS) have epidemiological, symptomatic and genetic overlap. Many people with IBD develop AS and vice versa, and both groups of patients exhibit pathological inflammation. This suggests a role for the immune system in linking the diseases. The aim of this study was to investigate the balance of macrophage and T cell subsets in the intestinal mucosa of people with IBD, AS, and healthy people. This was to improve our understanding of how innate and adaptive immune responses contribute to pathogenesis of inflammatory gut disorders. I hypothesised that genetic defects of the innate immune system, in people with IBD and AS, cause a shift in the proportion of macrophage subsets. Further, this would lead to chronic intestinal inflammation, characterised by IL-17 and IFNγ producing T cells. Methods were developed to identify complex T cell and macrophage subsets within human intestinal tissue. Multi-parametric flow cytometry was used to establish these methods, which were subsequently extended. For T cells, additional cytokines with relevance to IBD and AS were incorporated. This allowed detection of an increased frequency of T cells in the terminal ileum compared to the colon. For macrophages, additional phenotypic markers were incorporated to identify gut-specific macrophage subsets. Intestinal T cell profiles of people with CD, and AS were compared to those of healthy people. This revealed that people with inactive CD, and AS had an intermediate T cell phenotype between actively inflamed tissue and healthy tissue. This profile was characterised by differences in frequencies of IL-2 producing T cells and regulatory T cells. IL-17 producing T cells and regulatory T cells were increased in inflamed tissue compared to healthy, while IFNγ producing T cells were not. These immunological data, in combination with genetic studies, further elucidate how the immune system may be altered by genetic polymorphisms that lead to the development and maintenance of inflammatory diseases, such as IBD and AS. Based on the data presented here, I hypothesise that combinations of multiple genetic polymorphisms lower the threshold for development of intestinal inflammation, which leads to initiation of inflammatory diseases, including IBD and AS.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectIBD
dc.subjectAS
dc.subjectinflammatory bowel disease
dc.subjectankylosing spondylitis
dc.subjectimmune cells
dc.subjectT cell
dc.subjectmacrophage
dc.subjectflow cytometry
dc.titleIt all starts in the gut: The intestinal immune cell infiltrate in inflammatory bowel disease and ankylosing spondylitis
dc.typeThesis
dc.date.updated2014-06-26T02:20:21Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanno
otago.openaccessAbstract Only
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