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dc.contributor.advisorKirman, Joanna
dc.contributor.authorManners, Kate Meredith
dc.date.available2014-11-14T01:32:03Z
dc.date.copyright2014
dc.identifier.citationManners, K. M. (2014). The Role of Dendritic Cell Subsets in the Early Immune Response to Mycobacterial Infection (Thesis, Bachelor of Biomedical Sciences with Honours). University of Otago. Retrieved from http://hdl.handle.net/10523/5221en
dc.identifier.urihttp://hdl.handle.net/10523/5221
dc.description.abstractThe early immune response to tuberculosis (TB) is poorly understood. However, recent evidence has shown that dendritic cells (DC) are important for both control of bacterial growth and for activating adaptive immunity. DC are divided into functionally and phenotypically distinct subsets, but the role of each of these subsets in the early immune response to tuberculosis remains unknown. This study aimed to investigate the roles of DC subsets in a murine model of mycobacterial infection. Use of a fluorescent strain of the attenuated TB vaccine strain Mycobacterium bovis BCG, combined with multicolour flow cytometry, enabled detection of BCG associated DC subsets within the murine lung during the first 14 days of infection. The early immune response to mycobacterial infection was found to be highly dynamic, with significant variation in the proportion of BCG associated cells in each DC subset of the lung during infection. CD11b+ conventional DC (cDC) and plasmacytoid DC (pDC) were found to be associated with BCG during the first 14 days of infection, whereas CD103+ cDC were not. Differential expression of the cell surface markers GR-1, CD11b and CD11c detected within previously described DC subsets correlated with their propensity to associate with BCG. Conventional DC and pDC were found to respond differently to mycobacterial infection; cDC upregulated expression of CD86 during infection whereas pDC were found to do the opposite, suggesting that these subsets have distinct roles during early infection. Importantly, and contrary to current dogma, alveolar macrophages were not found to associate with BCG during early infection. This study highlights the distinct roles of different DC subsets during mycobacterial infection. This research may have implications for the development of a novel vaccine or therapy for tuberculosis, for which there is a significant need.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjecttuberculosis
dc.subjectmycobacteria
dc.subjectBCG
dc.subjectdendritic cell subsets
dc.subjectinnate immune response
dc.subjectflow cytometry
dc.subjectBCG tdTomato
dc.titleThe Role of Dendritic Cell Subsets in the Early Immune Response to Mycobacterial Infection
dc.typeThesis
dc.date.updated2014-11-14T01:13:55Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameBachelor of Biomedical Sciences with Honours
thesis.degree.grantorUniversity of Otago
thesis.degree.levelHonours
otago.interloanno
otago.openaccessAbstract Only
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