Pathology collection - Dunedin
Recent Deposits
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Profiling the pregnancy-specific glycoprotein genes in pre-eclampsia.
Background: Pre-eclampsia (PE) is a hypertensive disorder of pregnancy which is characterised by reduced placental cell invasion into the maternal arteries. Lack of invasion causes insufficient transformation of arteries ... -
Targeted Epigenetic Editing Using a dCas9- SunTag Methylation System
DNA methylation is a stable and somatically heritable epigenetic process. It is classically understood as a gene silencing mechanism, whereby hypermethylation of gene promoters is largely associated with transcriptional ... -
DNA methylation in pre-eclampsia: methodologies and analytics
The placenta is a heterogeneous and multifunctional organ that grows and adapts according to increasing and fluctuating fetal demands in the intrauterine environment. As expected, the placenta plays a major role in pregnancy ... -
Investigating the Direct Role of Promoter DNA Methylation in Gene Expression using a Targeted TET1CD-dCas9 Demethylase Tool
DNA hypermethylation of gene promoter regions has long been associated with transcriptional repression. Furthermore, the establishment of dense promoter methylation within tumour- suppressor genes has been widely-characterised ... -
In situ expression of biomarkers in placenta in the context of hypertensive disorders of pregnancy
Overview: Hypertensive disorders in pregnancy (HDP), of which the most common is pre- eclampsia (PE), are a leading cause of maternal and foetal morbidity and mortality worldwide. Despite extensive research, no clear cause ... -
Understanding the Cellular Consequences of Meier-Gorlin Syndrome
Meier-Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterised by short stature, microtia and patella aplasia/hypoplasia. Genetic variants, which cause MGS, have previously been found in genes involved ... -
Examining the role of cohesin subunit Rad21 in malignancy and development
The cohesin complex is essential for cell survival, owing to its well-established roles in cell division, DNA repair and gene expression. Malignancy and developmental disorders termed the cohesinopathies can result when ... -
Mechanisms of DNA demethylation
While every cell in an organism is genetically identical, there are marked phenotypic differences between tissues and organs that are controlled by epigenetic modifications. These epigenetic modifications provide an important ... -
Constitutive PD-L1 expression in melanoma
Treatment of melanoma based on targeted therapy and immunotherapy has dramatically advanced over the past decade. Advances in targeted therapy have been based on inhibition of the MAPK pathway while for immunotherapy, ... -
Investigating the Influence of Metabolic Syndrome on Cancer Immunotherapy
Cancer is a significant cause of morbidity in New Zealand, with breast and colorectal cancers being amongst the most commonly diagnosed. Therapeutic research has moved into immunotherapies, harnessing the immune system to ... -
Modeling RECQL4 developmental syndromes in Xenopus laevis using CRISPR/Cas9
There are a number of human genetic conditions of abnormal skeletal development associated with disrupted DNA replication, with several linked to mutations in RECQL4. While several mouse models targeting Recql4 have been ... -
Development of zebrafish models for leukaemia
Throughout development, numerous binary cell fate decisions are made where cells activate competing transcriptional networks and subsequently upregulate one network over others based on extrinsic cues. Cell fate decisions ... -
Identification of synthetic lethal compounds targeting cancers with cohesin mutations
The cohesin complex, comprising four core subunits, RAD21, SMC3, SMC1A, and STAG1/2, is important for the regulation of many biological processes, including sister chromatid cohesion, regulation of gene expression, chromatin ... -
Investigation into the relative location of filamin B and YB-1 in cell cycle progression
The cell cycle is a fundamental process in development and the maintenance of homeostasis. Various proteins regulate the cell cycle, such as Y-box binding protein -1 (YB-1). YB-1 transcriptionally regulates cell cycle ... -
Targeted Epigenetic Editing using a dCas9-SunTag Demethylation system
DNA methylation is a stable epigenetic modification, playing a critical role in the regulation of gene expression. Dysregulation of DNA methylation is implicated in the pathogenesis of numerous diseases including cancer. ... -
Regulation of Antigen Presenting Cells by Human Papillomavirus Small Extracellular Vesicles
Human Papillomavirus (HPV) causes over 99% of cervical cancer cases in the world, and of these, over 70% of cases can be attributed to high-risk HPV. There are prophylactic vaccines for HPV, that specifically target high-risk ... -
TP53 in renal vasculitis
Overview: Vasculitis is an autoimmune disease characterised by the swelling and inflammation of blood vessels. Renal vasculitis is a rare, yet severe disease characterised by the inflammation of glomeruli and affiliated ... -
Characterising the Functional and Molecular Determinants for Organ Selective Metastasis in Triple Negative Breast Cancer
Triple-negative breast cancer (TNBC) accounts for 10-20% of primary invasive breast cancer diagnoses, globally. With a higher risk of developing visceral and brain metastasis than other breast cancer subtypes, and limited ... -
The Expression of a p53 Isoform Δ133p53, in the Cell Cycle
Overview: p53 is a principal tumour suppressor protein that regulates the cell cycle. By doing so, p53 prevents damaged cells from undergoing DNA replication and cell division by activating pathways involved in DNA repair, ... -
Human papillomavirus gene expression in the placenta and its prevalence in an assisted reproduction cohort
Overview: Human papillomavirus (HPV) is well known as the leading cause of cervical cancer worldwide. Whether HPV also contributes to other adverse effects such as those associated with pregnancy is unclear. Higher incidences ...