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dc.contributor.advisorBunn, Stephen
dc.contributor.authorSreenivasan, Dharshini
dc.date.available2015-09-09T22:11:33Z
dc.date.copyright2015
dc.identifier.citationSreenivasan, D. (2015). The interaction of interleukin-6 with bovine adrenal medullary chromaffin cells (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/5874en
dc.identifier.urihttp://hdl.handle.net/10523/5874
dc.description.abstractThe aim of this study was to characterize the action of the cytokine interleukin-6 (IL-6) on the chromaffin cells of the adrenal medulla. These cells are traditionally recognized for their role in the neuronally mediated ‘fight-flight’ response and also respond to a range of other stressful stimuli by secreting both catecholamines and neuropeptides. A relationship between the immune and stress systems is likely to be of physiological importance and the failure of this interaction may contribute to a number of pathologies. Bovine chromaffin cells were isolated, purified and cultured on collagen-coated wells. Cells were then washed with physiological buffer and incubated with IL-6 for 5-30 min. Western blotting was employed to detect the activation of specific signalling proteins, the blots scanned and comparisons made between basal and IL-6 stimulated samples. IL-6 significantly increased the phosphorylation of signal transducer and activator of transcription-3 and extracellular signal-related kinases 1/2 but not p-38 and Jun amino-terminal kinase. Immunohistochemistry showed that approximately 70% of chromaffin cells had phospho-STAT3 within their nuclei the distribution of phospho-ERK1/2 was predominantly within the cytoplasm of these cells. Cells were also incubated with IL-6 for 48 h and quantitative-PCR used to identify the gene changes in selected neuropeptides in these cells. IL-6 caused an increase in the mRNA of the following peptides: galanin, secretogranin II and pro-enkephalin. The data obtained provides convincing evidence that the neuroendocrine chromaffin cells are responsive to IL-6 thus suggesting a potential pathway linking immune signals with the adrenal stress response.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectcytokine
dc.subjectinterleukin-6
dc.subjectbovine adrenal chromaffin cells
dc.subjectstress response
dc.subjectimmune response
dc.subjectcell signalling
dc.titleThe interaction of interleukin-6 with bovine adrenal medullary chromaffin cells
dc.typeThesis
dc.date.updated2015-09-09T21:23:01Z
dc.language.rfc3066en
thesis.degree.disciplineDepartment of Anatomy
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanyes
otago.openaccessAbstract Only
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