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dc.contributor.advisorFleming, Nicholas
dc.contributor.advisorBraithwaite, Antony
dc.contributor.authorSaha, Elora Konok
dc.date.available2015-10-14T23:10:19Z
dc.date.copyright2015
dc.identifier.citationSaha, E. K. (2015). Mutant p53 and pro-inflammatory gene expression in colorectal cancer cells (Thesis, Bachelor of Biomedical Sciences with Honours). University of Otago. Retrieved from http://hdl.handle.net/10523/5956en
dc.identifier.urihttp://hdl.handle.net/10523/5956
dc.description.abstractp53 gene is the most commonly mutated gene in cancer, and in many malignancies a loss of p53 promotes cancer progression. However recent evidence has shown that missense mutations in p53, particularly those that lead to the protein acquiring abilities not seen in the wild-type form, may actually aid oncogenesis. These so-called ‘gain of function’ mutations are thought to upregulate many pro-inflammatory cytokines, which can enhance tumour growth, and inhibit other proteins, such as TP63 – which has been shown to have anti-oncogenic properties. The central question of this project is to establish whether gain-of-function mutations in p53 influence the expression of pro-inflammatory genes in colorectal cancer (CRC) cells. To address this question, shRNA constructs against MDM2 were generated to stablise mutant p53, which were then transfected into various CRC cell lines that either harboured a gain-of-function mutation, had wildtype p53, or were void of all p53. In addition, a puromycin-resistance gene was included allowing for the selection of positively transfected cells using puromycin. qPCR primer sets were also developed for measuring the expression of several selected chemokines once the CRC lines had been transfected. To assess the ability of mutant p53 to functionally interact with p63, I attempted to generate a puromycin-selectable p63 expression construct. Resulting data suggest that gain-of-function p53 mutations may indeed heighten expression of various chemokines.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectmutant p53
dc.subjectcolorectal cancer
dc.subjectMDM2 knockdown
dc.subjectinflammatory signals
dc.subjectpathology
dc.subjectcancer
dc.titleMutant p53 and pro-inflammatory gene expression in colorectal cancer cells
dc.typeThesis
dc.date.updated2015-10-14T21:28:21Z
dc.language.rfc3066en
thesis.degree.disciplinePathology
thesis.degree.nameBachelor of Biomedical Sciences with Honours
thesis.degree.grantorUniversity of Otago
thesis.degree.levelHonours
otago.interloanno
otago.openaccessAbstract Only
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