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dc.contributor.advisorKemp, Roslyn
dc.contributor.advisorHook, Sarah
dc.contributor.authorHighton, Andrew John
dc.date.available2015-12-21T20:12:30Z
dc.date.copyright2015
dc.identifier.citationHighton, A. J. (2015). Immunisation with chitosan hydrogel generates CD8+ memory T cells protective against tumour challenge (Thesis, Doctor of Philosophy). University of Otago. Retrieved from http://hdl.handle.net/10523/6124en
dc.identifier.urihttp://hdl.handle.net/10523/6124
dc.description.abstractVaccination is used in the prevention of disease around the world. Through activation of T and B cells, and subsequent generation of memory cells, long lasting protection against disease is given. The majority of vaccines in use today exert their protective effect through generation of an antibody mediated response. It has been demonstrated that CD8+ T cells are important in protection against many diseases, including HIV and cancer. Modern vaccines able to generate an effective CD8+ memory T cell response may provide protection against these diseases. In this research, the sustained release vaccine vehicle chitosan hydrogel was assessed in its ability to generate an effective population of CD8+ memory T cells. It was hypothesised that vaccination of mice would generate a population of CD8+ memory T cells that were functional and protective against tumour challenge. Using flow cytometry, CD8+ memory T cells were identified in peripheral and gut associated lymphoid tissues of vaccinated mice. Furthermore, vaccination with chitosan hydrogel provided protection against both subcutaneous tumour challenge and in an orthotopic mouse model of colorectal cancer. Chitosan hydrogel represents a novel method of vaccine delivery. While it may be more suited towards vaccination against cancer, its ability to generate a cytotoxic immune response mediated by CD8+ T cells may be invaluable against some viral and bacterial infections with no current and effective prophylactic treatment. Thus, chitosan hydrogel is another step in modern vaccine delivery systems towards protection against a broader range of diseases and further research towards its efficacy in further tumour and infection disease models is warranted.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectImmunology
dc.subjectVaccine
dc.subjectT cell
dc.subjectCD8
dc.subjectCancer
dc.titleImmunisation with chitosan hydrogel generates CD8+ memory T cells protective against tumour challenge
dc.typeThesis
dc.date.updated2015-12-21T13:58:09Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameDoctor of Philosophy
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.interloanno
otago.openaccessAbstract Only
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