Abstract:
[…] The major objectives of this study were to determine more precisely the degree of relationship of rotaviral isolates from different host species by comparison at the genetic level. Before this could be fully investigated, suitable methods for the detection of rotavirus in a large number of specimens from a number of different host species had to be developed. Part II will describe the development of these techniques and particularly the enzyme-linked immunosorbant assay (ELISA). Part III will describe the extraction and comparison of genetic dsRNA from rotaviral isolates by polyacrylamide gel electrophoresis and the more precise comparison of this genetic material by molecular hybridization will be described in Part IV.
