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dc.contributor.advisorLove, Robert
dc.contributor.advisorMonk, Brian
dc.contributor.authorDevi, Arpana Arthi
dc.date.available2016-11-11T00:57:17Z
dc.date.copyright2016
dc.identifier.citationDevi, A. A. (2016). Enhancing the efficacy of antimicrobial peptide BM2, against mono-species biofilms, by combining with detergents. (Thesis, Doctor of Clinical Dentistry). University of Otago. Retrieved from http://hdl.handle.net/10523/6919en
dc.identifier.urihttp://hdl.handle.net/10523/6919
dc.description.abstractAim To investigate if a detergent regime could enhance the antimicrobial ability of BM2. Method Strains of Enterococcus faecalis, Streptococcus gordonii, Streptococcus mutans, and Candida albicans were grown from glycerol stocks after confirmation of the strains. After subculturing single colonies were cultured in TSB and CSM liquid media for 24hr to obtain a microbial suspension which was adjusted to OD600nm = 0.5. Dilution series of the peptidomimetic BM2 and detergents were prepared in aqueous solution and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a broth micro-dilution method. Further on planktonic cells and monospecies biofilms were exposed to the detergent and BM2 combinations. The efficacy of BM2 and detergents at causing biofilm detachment was measured using a crystal violet based assay. Results Planktonic cells were easier to kill with some of the detergents in isolation or in combination with BM2. SDS and CTAB in combination with BM2 increased the efficacy of BM2 against the test organisms. Tween 20 did not kill any of the test organisms alone or in combination. Biofilms were harder to eradicate and detergent, BM2 combinations gave varied results for the different species tested. Detergents in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting S. mutans biofilm. SDS in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting E. faecalis biofilm at the 24 and 48 hr periods however at the 72 hr period there was disruption of biofilm. CTAB in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting E. faecalis biofilm. iii CTAB in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting S. gordonii biofilm at the 24 hr period, however at the 48 and 72 hr no disruption was noted. SDS in combination with BM2 did not increase the efficacy of the antimicrobial peptide in disrupting S. gordonii biofilm. CTAB in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting C. albicans biofilm at the 24 hr period, however at the 48 and 72 hr no disruption was noted. SDS in combination with BM2 increased the efficacy of the antimicrobial peptide in disrupting C. albicans biofilm at the 24 and 48 hr periods, however at the 72 hr period, the combination did not disrupt C. albicans biofilm. Conclusion Within the limits of this in-vitro study a combination of BM2 and SDS showed antimicrobial action against certain endodontic microorganisms occurring as planktonic cells and in biofilms and offers the potential to develop an effective between visits’ medicament.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectAntimicrobial
dc.subjectpeptide
dc.subjectbiofilm
dc.subjectEndodontics
dc.subjectdetergents
dc.titleEnhancing the efficacy of antimicrobial peptide BM2, against mono-species biofilms, by combining with detergents.
dc.typeThesis
dc.date.updated2016-11-10T23:57:09Z
dc.language.rfc3066en
thesis.degree.disciplineDepartment of Oral rehabilitation
thesis.degree.nameDoctor of Clinical Dentistry
thesis.degree.grantorUniversity of Otago
thesis.degree.levelDoctoral
otago.openaccessOpen
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