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dc.contributor.advisorGeorge, Peter
dc.contributor.advisorJensen, Berit
dc.contributor.authorDowning, Kim
dc.date.available2017-11-02T21:16:03Z
dc.date.copyright2017
dc.identifier.citationDowning, K. (2017). The Analysis and Stability of Carboplatin (Thesis, Master of Medical Laboratory Science). University of Otago. Retrieved from http://hdl.handle.net/10523/7680en
dc.identifier.urihttp://hdl.handle.net/10523/7680
dc.description.abstractCarboplatin is a chemotherapy drug used in a variety of cancers with the primary toxicity being exposure-dependant myelosuppression. We present the development and validation of a simple, robust inductively coupled plasma mass spectrometry (ICP-MS) method to measure carboplatin in plasma ultrafiltrate. Plasma ultrafiltrates samples were prepared using Amicon Ultra 30,000da cut-off filters and then diluted with ammonia EDTA before ICP-MS analysis. The assay was validated in the range 0.19-47.5 mg/L carboplatin in ultrafiltrate. The assay was linear (r2>0.9999), accurate (<6% bias, 12% bias at LLOQ) and precise (intra- and inter-day precision of <3% coefficient of variation). No matrix effects were observed between plasma ultrafiltrate and aqueous platinum calibrators and recovery was complete. The assay was applied to 10 clinical samples from patients receiving carboplatin. Incurred sample reanalysis showed reproducible values over 3 analysis days (<6% CV). As plasma stability prior to ultrafiltration has been a major concern in previous clinical studies this was studied extensively at room temperature (22°C) over 24 hours. Carboplatin was found to be stable in both spiked plasma (n=3) and real patient samples (n=10) at room temperature for up to 8 hours before ultrafiltration. This makes routine measurement of carboplatin concentrations in clinical settings feasible.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectCarboplatin
dc.subjectICP-MS
dc.subjectStability
dc.subjectPlatinum
dc.subjectPt
dc.subjectUltrafiltration
dc.subjectUltrafiltrate
dc.titleThe Analysis and Stability of Carboplatin
dc.typeThesis
dc.date.updated2017-11-02T21:09:38Z
dc.language.rfc3066en
thesis.degree.disciplinePathology
thesis.degree.nameMaster of Medical Laboratory Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanyes
otago.openaccessAbstract Only
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