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dc.contributor.advisorKemp, Roslyn
dc.contributor.advisorMcCall, John
dc.contributor.authorShen, Shirley
dc.date.available2017-11-02T21:55:36Z
dc.date.copyright2017
dc.identifier.citationShen, S. (2017). T cell signalling in patients with colorectal cancer (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/7682en
dc.identifier.urihttp://hdl.handle.net/10523/7682
dc.description.abstractT lymphocytes (T cells) are essential for mediating an effector response against tumours. High infiltrates of T cells in the tumour from colorectal cancer (CRC) patients are associated with positive patient outcomes, indicating their importance in the anti-tumour response in colorectal cancer. The survival and proliferation of T cell depends on the gamma chain family of cytokines, namely, interleukin (IL)-2, IL-7 and IL-15. Therefore, understanding the ability of T cells to respond to signals from these cytokines in cancer patients is essential. Phosphoflow cytometry is a method used to detect activation of signalling pathways by measuring downstream phosphorylation events. The aim of this study was to optimise the phosphoflow cytometry technique and use it to measure signal transducer and activator of transcription (STAT) 5 phosphorylation in T cells from patients with CRC and healthy controls in response to IL-2, IL-7, and IL-15 stimulation. This study found there was no significant difference in signalling through STAT5 phosphorylation in T cells from the blood of colorectal cancer patients compared to healthy controls, and in colorectal tumour tissue compared to non-tumour bowel tissue. However, although no significant differences in signalling were observed, the expression of the surface receptors CD127 and CD122 on CD4+ tumour infiltrating T cells was significantly decreased and increased, respectively, indicating that there may be downstream signalling abnormalities in the T cells. However, these results are preliminary and will need to be validated in a larger cohort of patients.
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectImmunology
dc.subjectcolorectal
dc.subjectcancer
dc.subjectimmune
dc.subjectsignalling
dc.subjectphosphoflow
dc.titleT cell signalling in patients with colorectal cancer
dc.typeThesis
dc.date.updated2017-11-02T07:46:10Z
dc.language.rfc3066en
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.nameMaster of Science
thesis.degree.grantorUniversity of Otago
thesis.degree.levelMasters
otago.interloanno
otago.openaccessAbstract Only
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