T cell signalling in patients with colorectal cancer
|dc.identifier.citation||Shen, S. (2017). T cell signalling in patients with colorectal cancer (Thesis, Master of Science). University of Otago. Retrieved from http://hdl.handle.net/10523/7682||en|
|dc.description.abstract||T lymphocytes (T cells) are essential for mediating an effector response against tumours. High infiltrates of T cells in the tumour from colorectal cancer (CRC) patients are associated with positive patient outcomes, indicating their importance in the anti-tumour response in colorectal cancer. The survival and proliferation of T cell depends on the gamma chain family of cytokines, namely, interleukin (IL)-2, IL-7 and IL-15. Therefore, understanding the ability of T cells to respond to signals from these cytokines in cancer patients is essential. Phosphoflow cytometry is a method used to detect activation of signalling pathways by measuring downstream phosphorylation events. The aim of this study was to optimise the phosphoflow cytometry technique and use it to measure signal transducer and activator of transcription (STAT) 5 phosphorylation in T cells from patients with CRC and healthy controls in response to IL-2, IL-7, and IL-15 stimulation. This study found there was no significant difference in signalling through STAT5 phosphorylation in T cells from the blood of colorectal cancer patients compared to healthy controls, and in colorectal tumour tissue compared to non-tumour bowel tissue. However, although no significant differences in signalling were observed, the expression of the surface receptors CD127 and CD122 on CD4+ tumour infiltrating T cells was significantly decreased and increased, respectively, indicating that there may be downstream signalling abnormalities in the T cells. However, these results are preliminary and will need to be validated in a larger cohort of patients.|
|dc.publisher||University of Otago|
|dc.rights||All items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.|
|dc.title||T cell signalling in patients with colorectal cancer|
|thesis.degree.discipline||Microbiology and Immunology|
|thesis.degree.name||Master of Science|
|thesis.degree.grantor||University of Otago|
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