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dc.contributor.advisorAugustine, Rachael
dc.contributor.authorMurrell, Chantelle
dc.date.available2020-03-03T01:50:02Z
dc.date.copyright2020
dc.identifier.citationMurrell, C. (2020). Expression of O-linked N-acetylglucosamine in the Rat Brain during Pregnancy and Lactation (Thesis, Bachelor of Biomedical Sciences with Honours). University of Otago. Retrieved from http://hdl.handle.net/10523/9922en
dc.identifier.urihttp://hdl.handle.net/10523/9922
dc.description.abstractDuring pregnancy, adaptations occur in the maternal brain and body to ensure optimal nutrition for both the mother and fetus. Over nutrition in the mother can lead to gestational diabetes mellitus (GDM). GDM in mothers is a significant risk factor for development of cardiovascular disease and pre-eclampsia (Carr et al.,2011). The protein modification O-linked N-acetylglucosamine (O-GlcNAc) is derived from glucose through the hexosamine biosynthetic pathway (HBP) and has been shown to increase in hyperglycemic conditions and plays a role in the etiology of many diseases, including T2DM (Hart et al., 2011). Research on the changes of O-GlcNAcylation during pregnancy and lactation is scarce. My study aimed to determine any changes in O-GlcNAc expressing cells during the physiological conditions of pregnancy and lactation in the rat brain. I hypothesised that there would be an increased number of O-GlcNAc expressing cells during pregnancy due to higher blood glucose levels, which would normalise or decrease during lactation compared to non-pregnant rats. Female Sprague Dawley rats were divided into 3 groups (diestrus, 21 days pregnant and day 7 lactating), perfused with 4% paraformaldehyde, brains removed and coronally sliced for immunohistochemistry. Single-label chromogen staining for O-GlcNAc was carried out in the ventromedial hypothalamus (VMH), paraventricular nucleus (PVN), supraoptic nucleus (SON), periventricular nucleus (Pe) and the anteroventral periventricular nucleus (AVPV). Single-label immunofluorescence for O-GlcNAc was carried out in the arcuate nucleus (ARC). Sections were photographed and analysed using FIJI (Image J) to count the number of O-GlcNAc expressing cells in each area. There was no statistical difference between the number of O-GlcNAc expressing cells in pregnant and lactating rats compared with non-pregnant rats in the ARC, PVN, SON, VMH, Pe and AVPV. Double-label immunofluorescence for O-GlcNAc and DeltaFosB, a marker of chronic activation, was also carried out in the PVN. The average number of DeltaFosB expressing cells and O-GlcNAc expressing cells were counted, no difference was detected between the non-pregnant, pregnant and lactating groups. Furthermore, the percentage of DeltaFosB cells co-expressing O-GlcNAc and the percentage O-GlcNAc cells co-expressing DeltaFosB revealed no difference between the non-pregnant, pregnant and lactating groups. The results from our study suggests that in a normal rat pregnancy and subsequent lactation the expression of O-GlcNAc does not differ from non-pregnant expression.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherUniversity of Otago
dc.rightsAll items in OUR Archive are provided for private study and research purposes and are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectO-GlcNAc
dc.subjectO-linked N-acetylglucosamine
dc.subjectRat Brain
dc.subjectPregnancy
dc.subjectLactation
dc.titleExpression of O-linked N-acetylglucosamine in the Rat Brain during Pregnancy and Lactation
dc.typeThesis
dc.date.updated2020-02-16T21:45:59Z
dc.language.rfc3066en
thesis.degree.disciplinePhysiology
thesis.degree.nameBachelor of Biomedical Sciences with Honours
thesis.degree.grantorUniversity of Otago
thesis.degree.levelHonours
otago.openaccessOpen
otago.evidence.presentYes
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