Abstract
Pseudokinases are a class of kinase-related proteins that lack catalytic activity. They are involved in many cellular signalling pathways, and as such, their dysregulation has been implicated in wide range of diseases. In mouse models, overexpression of the pseudokinase TRIB2 has been linked to the development of acute myeloid leukemia through inactivation of the transcription factor C/EBPα. TRIB2 is known to be somewhat unstable, which makes it challenging to elucidate its three-dimensional structure.
The aim of this project was to use biochemical techniques to identify TRIB2-binding nanobodies (NBs) that could stabilise TRIB2 and thus aid structure determination by x-ray crystallography.