Abstract
Interleukin 10 (IL-10) is a dynamic cytokine produced by most adaptive and innate immune cells. Although IL-10 is known as a potent anti-inflammatory cytokine, it can also stimulate the activity of B cells, cytotoxic T cells, natural killer cells and mast cells. This has hindered the use of IL-10 as a therapeutic in immune mediated diseases. This project aimed to identify IL-10 mutants that exhibit signalling bias to develop as selective anti-inflammatory therapies. We hypothesized that structurally-guided human IL-10 mutants with altered receptor affinity could bias IL-10 signalling towards therapeutic immune suppressive pathways.