Abstract
Migraine is a debilitating neurological disorder, and a leading cause of disability worldwide. Calcitonin gene-related peptide (CGRP) is pathologically linked to migraine and potently activates two receptors, the canonical CGRP receptor and the Amylin 1 (AMY1) receptor. It is unknown to what extent AMY1 is responsible for the biological action of CGRP, as research has so far focused on the CGRP receptor. A key question is whether CGRP and AMY1 are co-expressed in migraine relevant brain regions. This study therefore investigated the relative localisation of CGRP and the calcitonin receptor (CTR) subunit of the AMY1 receptor in the dorsolateral pons, a region containing two key structures relevant to migraine, the locus coeruleus (LC) and parabrachial nucleus (PBN).