Abstract
The clinical features of many genetic disorders are often caused by complex mechanisms that are poorly understood. Recently, mutations in DONSON, a DNA replication protein, have been found to cause microcephaly (reduced brain size) in children. The developmental processes that cause this reduction in size remain unknown. This project aimed to study the effects of disrupting Donson using the CRISPR Cas9 editing tool in the developing brain of Xenopus laevis (African clawed frog).